Lu H, Ye M Q, Thung S N, Dash S, Gerber M A
Department of Pathology and Laboratory Medicine, School of Medicine, Tulane University, 1430 Tulane Avenue, New Orleans, Louisiana 70112, USA.
Chin Med J (Engl). 2000 Dec;113(12):1138-41.
To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied.
RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting.
Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%).
Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.
研究丙型肝炎病毒（HCV）在胆管细胞恶性转化中的作用。对6例中国胆管癌患者和6例美国胆管癌患者的组织进行了研究。
从福尔马林固定、石蜡包埋切片的选定肿瘤区域提取RNA，随后进行逆转录双重聚合酶链反应（RT-PCR）和Southern印迹分析。
12例胆管癌患者中有7例检测到正链和负链HCV RNA序列。与美国患者（33%）相比，中国患者的阳性率较高（83%）。
我们的研究结果表明HCV可能在胆管细胞的恶性转化中起作用。
