Yin F, Chen B
Department of Surgery, Fujian Medical University Union Hospital, Inistitute of Hepatobiliary Surgery, Fuzhou 350001.
Chin Med J (Engl). 1998 Dec;111(12):1068-70.
To detect hepatitis C virus (HCV) RNA sequences in the hepatic portal cholangiocarcinoma tissues and their relationship.
RNA was extracted from paraffin-embedded cholangiocarcinoma tissues of 6 patients by guanidinium method, subjected to reverse transcription and then amplified by double PCR technique using nested primers from the highly conserved 5' noncoding region of HCV genome.
HCV RNA of 5' NT sequences was found in the hepatic portal cholangiocarcinoma tissues of 5 out of 6 (83%) patients.
HCV RNA sequences present with high infectious rate in cholangiocarcinoma, and reverse transcription polymerase chain reaction (RT-PCR) assay using primers derived from 5' NT region of HCV sequence is most useful in detecting HCV infection. The development of cholangiocarcinoma awaits further studies.
检测肝门部胆管癌组织中丙型肝炎病毒（HCV）RNA序列及其相互关系。
采用胍盐法从6例患者石蜡包埋的胆管癌组织中提取RNA，进行逆转录，然后使用来自HCV基因组高度保守的5'非编码区的巢式引物通过双重PCR技术进行扩增。
6例患者中有5例（83%）的肝门部胆管癌组织中发现了5'NT序列的HCV RNA。
HCV RNA序列在胆管癌中具有较高的感染率，使用源自HCV序列5'NT区域的引物进行逆转录聚合酶链反应（RT-PCR）检测对HCV感染最为有用。胆管癌的发病机制有待进一步研究。
