Kanesaki Yu, Suzuki Iwane, Allakhverdiev Suleyman I, Mikami Koji, Murata Norio
Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Biochem Biophys Res Commun. 2002 Jan 11;290(1):339-48. doi: 10.1006/bbrc.2001.6201.
Acclimation of microorganisms to environmental stress is closely related to the expression of various genes. We report here that salt stress and hyperosmotic stress have different effects on the cytoplasmic volume and gene expression in Synechocystis sp. PCC 6803. DNA microarray analysis indicated that salt stress strongly induced the genes for some ribosomal proteins. Hyperosmotic stress strongly induced the genes for 3-ketoacyl-acyl carrier protein reductase and rare lipoprotein A. Genes whose expression was induced both by salt stress and by hyperosmotic stress included those for heat-shock proteins and the enzymes for the synthesis of glucosylglycerol. We also found that each kind of stress induced a number of genes for proteins of unknown function. Our findings suggest that Synechocystis recognizes salt stress and hyperosmotic stress as different stimuli, although mechanisms common to the responses to each form of stress might also contribute to gene expression.
微生物对环境胁迫的适应与各种基因的表达密切相关。我们在此报告，盐胁迫和高渗胁迫对集胞藻PCC 6803的细胞质体积和基因表达有不同影响。DNA微阵列分析表明，盐胁迫强烈诱导了一些核糖体蛋白基因的表达。高渗胁迫强烈诱导了3-酮酰基-酰基载体蛋白还原酶和稀有脂蛋白A基因的表达。盐胁迫和高渗胁迫均诱导表达的基因包括热休克蛋白基因和葡糖基甘油合成酶基因。我们还发现，每种胁迫都诱导了许多功能未知的蛋白质基因的表达。我们的研究结果表明，集胞藻将盐胁迫和高渗胁迫识别为不同的刺激，尽管对每种胁迫形式的反应中共同的机制也可能有助于基因表达。
