Tsuchiya Masahiko, Asada Akira, Kasahara Emiko, Sato Eisuke F, Shindo Mitsuo, Inoue Masayasu
Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Abenoku, Osaka, Japan.
Am J Respir Crit Care Med. 2002 Jan 1;165(1):54-60. doi: 10.1164/ajrccm.165.1.2010134.
alpha-Tocopherol is a potent antioxidant that effectively protects biological membranes against oxidative injury through coordination with ascorbic acid. Because propofol has a phenolic structure similar to that of alpha-tocopherol, this intravenous anesthetic may also have similar antioxidant activity. To test this hypothesis, the effect of propofol on oxidative injury of human erythrocytes was examined. Propofol inhibited oxidative hemolysis and cis-parinaric acid oxidation in erythrocyte membranes (ED(50) = 6 microM). Although ascorbic acid alone has no appreciable effect, the protective effect of propofol was enhanced by ascorbic acid. An electron spin resonance (ESR) study showed that propofol-derived radicals (g = 2.005) were continuously generated during the oxidation of erythrocyte membranes by an ascorbic acid-inhibitable mechanism. These and other results suggest that propofol interacts with ascorbic acid, thereby exhibiting potent antioxidant activity in and around membranes as does alpha-tocopherol. Kinetic analysis revealed that propofol increased the membrane fluidity of erythrocytes, thereby increasing their resistance to physical and hemodynamic stress. Further, a greater preservation of red blood cell counts was seen after surgery with propofol compared with conventional sevoflurane anesthesia. Thus, propofol may protect erythrocytes against both oxidative and physical stress, indicating its potential as an efficient and safe antioxidant.
α-生育酚是一种有效的抗氧化剂，它通过与抗坏血酸协同作用，有效保护生物膜免受氧化损伤。由于丙泊酚具有与α-生育酚相似的酚类结构，这种静脉麻醉剂可能也具有类似的抗氧化活性。为了验证这一假设，研究了丙泊酚对人红细胞氧化损伤的影响。丙泊酚抑制红细胞膜中的氧化溶血和顺式-十八碳三烯酸氧化（半数有效剂量=6微摩尔）。虽然单独的抗坏血酸没有明显作用，但抗坏血酸增强了丙泊酚的保护作用。电子自旋共振（ESR）研究表明，在红细胞膜氧化过程中，丙泊酚衍生的自由基（g=2.005）通过一种抗坏血酸可抑制的机制持续产生。这些以及其他结果表明，丙泊酚与抗坏血酸相互作用，从而在膜内外表现出与α-生育酚一样强大的抗氧化活性。动力学分析表明，丙泊酚增加了红细胞的膜流动性，从而增加了它们对物理和血液动力学应激的抵抗力。此外，与传统的七氟醚麻醉相比，丙泊酚麻醉术后红细胞计数的保留情况更好。因此，丙泊酚可能保护红细胞免受氧化和物理应激，表明其作为一种高效且安全的抗氧化剂的潜力。
