Vargas-Alarcón G, Londoño J D, Hernández-Pacheco G, Gamboa R, Castillo E, Pacheco-Tena C, Cardiel M H, Granados J, Burgos-Vargas R
Cellular Biology Section, Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Tlalpan, Mexico DF, Mexico.
Ann Rheum Dis. 2002 Jan;61(1):48-51. doi: 10.1136/ard.61.1.48.
To investigate the role of HSP70 genes as contributors to genetic susceptibility of the spondyloarthropathies (SpA) in the Mexican population.
The study included 150 patients with SpA (undifferentiated spondyloarthropathy (uSpA) 68, ankylosing spondylitis (AS) 60, and reactive arthritis 22) and 158 healthy controls. HSP70-1, HSP70-2 and HSP70-hom genotypes were analysed by the polymerase chain reaction-restriction fragment length polymorphism technique. Statistical methods included the Mantel-Haenzel, chi(2), Fisher's exact test, and Woolf's method for odds ratio (OR).
HSP70-2 B/B genotype frequency was increased in the whole group of patients with SpA (pC<0.05, OR=4.3), as well as in the different clinical subgroups (pC<0.05, OR=4.2 for AS; pC<0.05, OR=4.4 for uSpA; and pC<0.05, OR=4.1 for ReA). This frequency remained significantly increased when the patients with B27 negative SpA were analysed. On the other hand, HSP70-hom locus analysis showed significantly increased frequency of A allele in the whole group of SpA (pC<0.05, OR=3.4), as well as in the groups with AS (pC<0.05, OR=5.6) and with uSpA (pC<0.05, OR=3.1), when compared with healthy controls. In this case, also, the genotype A/A was increased in the whole group of SpA (pC<0.05, OR=4.5), as well as in patients with AS (pC<0.05, OR=6.4) and with uSpA (pC<0.05, OR=3.7). When the patients with B27 negative SpA were analysed the frequencies of HSP70-hom A allele and A/A genotype remained significantly increased in the whole group of SpA (pC<0.05, OR=3.2 for the A allele and pC<0.05, OR=4.2 for the A/A genotype) and in the uSpA subgroup (pC<0.05, OR=3.8 for the A allele and pC<0.05, OR=4.3 for the A/A genotype).
In addition to the association of SpA with HLA-B27, there is a significant association of HSP70-2 and HSP70-hom alleles with SpA in Mexicans. This association seems to be independent of the susceptibility conferred by HLA-B27 in the group of patients with uSpA.
研究热休克蛋白70（HSP70）基因在墨西哥人群脊柱关节病（SpA）遗传易感性中的作用。
本研究纳入150例SpA患者（未分化脊柱关节病（uSpA）68例、强直性脊柱炎（AS）60例、反应性关节炎22例）和158例健康对照。采用聚合酶链反应-限制性片段长度多态性技术分析HSP70-1、HSP70-2和HSP70-hom基因型。统计方法包括Mantel-Haenzel法、卡方检验、Fisher精确检验以及Woolf法计算比值比（OR）。
SpA患者全组中HSP70-2 B/B基因型频率升高（pC<0.05，OR=4.3），不同临床亚组中也是如此（AS组：pC<0.05，OR=4.2；uSpA组：pC<0.05，OR=4.4；反应性关节炎组：pC<0.05，OR=4.1）。分析B27阴性SpA患者时，该频率仍显著升高。另一方面，与健康对照相比，SpA患者全组中HSP70-hom基因座分析显示A等位基因频率显著升高（pC<0.05，OR=3.4），AS组（pC<0.05，OR=5.6）和uSpA组（pC<0.05，OR=3.1）也是如此。同样，SpA患者全组中A/A基因型频率升高（pC<0.05，OR=4.5），AS患者（pC<0.05，OR=6.4）和uSpA患者（pC<0.05，OR=3.7）也是如此。分析B27阴性SpA患者时，SpA患者全组（A等位基因：pC<0.05，OR=3.2；A/A基因型：pC<0.05，OR=4.2）和uSpA亚组（A等位基因：pC<0.05，OR=3.8；A/A基因型：pC<0.05，OR=4.3）中HSP70-hom A等位基因和A/A基因型频率仍显著升高。
除了SpA与HLA-B27的关联外，HSP70-2和HSP70-hom等位基因与墨西哥人的SpA存在显著关联。这种关联似乎独立于HLA-B27赋予uSpA患者组的易感性。
