Li F, Niu C, Ye B
Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China.
Chin Med J (Engl). 2001 Jun;114(6):654-7.
To detect malaria DNA in mosquitoes.
A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used.
In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121 bp band was seen with DNA templates from other malaria parasites or negative mosquitoes.
Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.
检测蚊子体内的疟疾DNA。
采用巢式聚合酶链反应（巢式PCR）程序，该程序可扩增间日疟原虫特异性小亚基核糖体RNA（SSUrRNA）基因的121 bp DNA。
在实验室感染的蚊子中，巢式PCR能够检测到低至3个孢子体，或在99只正常蚊子的群体中混入1只受感染蚊子。此外，来自其他疟原虫或阴性蚊子的DNA模板未出现特异性的121 bp条带。
所获得的敏感性和特异性表明，在检测低度寄生虫感染蚊子中的间日疟原虫孢子体方面，巢式PCR比DNA探针或直接PCR具有优势。
