尽管进行了免疫预防仍被感染的婴儿中的乙肝病毒S基因变异体

Hepatitis B virus S gene mutants in infants infected despite immunoprophylaxis.

作者信息

Zhu Q, Lu Q, Xiong S, Yu H, Duan S

机构信息

Department of Infectious Diseases, Children's Hospital, Medical Center of Fudan University, Shanghai 200032, China.

出版信息

Chin Med J (Engl). 2001 Apr;114(4):352-4.

PMID:11780452

Abstract

OBJECTIVE

To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure.

METHODS

Using sera from 106 infants who were born to HBV carrier mothers and failed in HB immunoprophylaxis, HBV S gene was amplified by PCR, transferred to nylon membranes for Southern blots, and then hybridized with oligonucleotide probes. Eleven of non-hybridizing samples were used for DNA sequencing.

RESULTS

93.4% (99/106) of the samples were HBV DNA positive, and 30.3% (30/99) failed to hybridize with at least one of the four probes. DNA sequencing confirmed that 10 of the 11 samples had an S gene mutation with amino acid (aa) change. The identified mutants included nucleotide (nt) 546T-->A (aa131N-->T), nt531T-->C (aa1261-->T), nt491A-->C (aa113T-->P), nt491T-->A (aa113S-->T), nt533C-->A (aa127P-->T), nt581T-->A (aa143S-->T), nt636A-->T (aa161Y-->F), and nt679A-->C (aa175L-->F). The sequence in one mother-infant pair was completely the same, with mutations at aa131 and aa161.

CONCLUSIONS

The prevalence of HBV surface mutants is about 30% in the children failing in HB vaccination. HBV mutants can infect infants by maternal-infant transmission.

摘要

目的

评估乙型肝炎病毒（HBV）表面基因突变感染与乙型肝炎（HB）疫苗接种失败之间的相关性。

方法

采用106名母亲为HBV携带者且HB免疫预防失败的婴儿的血清，通过聚合酶链反应（PCR）扩增HBV S基因，转移至尼龙膜进行Southern杂交，然后与寡核苷酸探针杂交。对11个未杂交的样本进行DNA测序。

结果

93.4%（99/106）的样本HBV DNA呈阳性，30.3%（30/99）的样本至少与四种探针中的一种未能杂交。DNA测序证实，11个样本中有10个发生了S基因突变且氨基酸（aa）发生改变。鉴定出的突变包括核苷酸（nt）546T→A（aa131N→T）、nt531T→C（aa126I→T）、nt491A→C（aa113T→P）、nt491T→A（aa113S→T）、nt533C→A（aa127P→T）、nt581T→A（aa143S→T）、nt636A→T（aa161Y→F）和nt679A→C（aa175L→F）。一对母婴的序列完全相同，在aa131和aa161处发生突变。