Ma Chun-Ling, Fang De-Xing, Yao Kun, Li Fa-Qing, Jin Hui-Ying, Li Su-Qin, Tan Wei-Guo
Huadong Research Institute for Medical Biotechnics, Nanjing 210029, Jiangsu Province, China.
World J Gastroenterol. 2005 Jan 14;11(2):299-302. doi: 10.3748/wjg.v11.i2.299.
To investigate the epidemiology of hepatitis B virus (HBV) strains with a mutation at nt551 in surface gene among hepatitis B patients in Nanjing and its neighbourhood.
By using mutation-specific polymerase chain reaction (msPCR) established by our laboratory for amplifying HBV DNAs with a mutation at nt551, 117 serum samples taken from hepatitis B patients were detected.
The results showed that 112 samples were positive for nt551A, 4 samples were positive for nt551G. One sample was positive for nt551T. No nt551C of HBV DNA was found. The incidence of HBsAg mutants with G, C, T, A at nt551 among 117 samples was 3.42%, 0%, 0.85%, 95.73%, respectively.
In Nanjing and its neighbourhood, hepatitis B patients are mainly infected with wild genotype HBV. The incidence of mutants with a mutation at nt551 in HBV genome is significantly lower than that in wild genotype HBV DNA (P<0.01). The necessity of adding components of HBsAg mutants to HBV vaccine needs further investigation.
调查南京及其周边地区乙肝患者中表面基因nt551位点发生突变的乙肝病毒（HBV）毒株的流行病学情况。
采用本实验室建立的突变特异性聚合酶链反应（msPCR）扩增nt551位点发生突变的HBV DNA，对117例乙肝患者的血清样本进行检测。
结果显示，112份样本nt551A阳性，4份样本nt551G阳性，1份样本nt551T阳性。未发现HBV DNA的nt551C。117份样本中，nt551位点G、C、T、A突变的HBsAg突变体发生率分别为3.42%、0%、0.85%、95.73%。
在南京及其周边地区，乙肝患者主要感染野生基因型HBV。HBV基因组nt551位点发生突变的突变体发生率显著低于野生基因型HBV DNA（P<0.01）。乙肝疫苗中添加HBsAg突变体成分的必要性有待进一步研究。
