Benedict R W, Williams M C
Biomater Med Devices Artif Organs. 1979;7(4):457-75. doi: 10.3109/10731197909118962.
Polypropylene (PP) disks activated by exposure to ammonia glow discharge were used as substrates for red cells deposited from saline suspensions. In some cases these cell-coated disks were further treated by glutaraldehyde to bind the cells more strongly. Each disk was used in a rotational blood-shearing device to induce hemolysis, which was compared with that induced by virgin PP. When the cell coating was uniform and dense from a single settling of cells, the glutaraldehyde-fixed surfaces were about 10% less hemolytic than PP. Non-fixed cells detached from outer regions of the disk and apparently contributed additional hemolysis in the process. Secondary layers of settled cells, both fixed and non-fixed, also proved to be more hemolytic than PP. Coatings of gamma-globulin reduced hemolysis relative to uncoated cell surfaces. The best performance was that of activated surfaces without cells, with hemolysis about 20% less than PP. Tests of cells hemolyzing during detachment in a saline medium suggested that hemolysis of whole blood involves a series of brief attachment/detachment events at the solid surface.
将暴露于氨辉光放电下活化的聚丙烯（PP）圆盘用作从盐水悬浮液中沉积红细胞的基质。在某些情况下，这些细胞包被的圆盘会进一步用戊二醛处理，以使细胞结合更牢固。每个圆盘都用于旋转式血液剪切装置中以诱导溶血，并将其与原始PP诱导的溶血进行比较。当细胞涂层通过单次细胞沉降均匀且致密时，戊二醛固定的表面溶血程度比PP低约10%。未固定的细胞从圆盘外部区域脱落，显然在这个过程中导致了额外的溶血。沉降细胞的第二层，无论是固定的还是未固定的，溶血程度也比PP更高。相对于未包被细胞的表面，γ-球蛋白包被减少了溶血。性能最佳的是没有细胞的活化表面，溶血程度比PP低约20%。在盐溶液介质中细胞脱离过程中溶血的测试表明，全血溶血涉及在固体表面发生的一系列短暂附着/脱离事件。
