Nichols A R, Williams M C
Biomater Med Devices Artif Organs. 1976;4(1):21-48. doi: 10.3109/10731197609118643.
Hemolysis was induced in erythrocyte suspensions by shearing between rotating metal disks 10 cm in diameter at 325 rev/min; stresses did not exceed 100 dynes/cm2. The role of proteins (gamma-globulin, G; albumin, A; fibrinogen, F) in the environment was tested in two ways. First, disks were precoated by exposure to protein solutions of physiologic concentration and then used to hemolyze the suspensions. Tests carried to 10(4) sec showed protective effects, relative to shearing with virgin disks, with a ranking G greater than A greater than F. Second, proteins were dissolved in the suspension media and hemolysis compared to the case without protein. Large suppression of hemolysis was found during storage (G approximately A greater than F) but only G offered significant protection against shear.
通过在直径10厘米的旋转金属盘之间以325转/分钟的速度剪切来诱导红细胞悬液发生溶血；应力不超过100达因/平方厘米。以两种方式测试了环境中蛋白质（γ球蛋白，G；白蛋白，A；纤维蛋白原，F）的作用。首先，通过将圆盘暴露于生理浓度的蛋白质溶液中进行预包被，然后用于使悬液溶血。进行到10⁴秒的测试显示，相对于使用未处理圆盘进行剪切，具有保护作用，其保护作用排序为G＞A＞F。其次，将蛋白质溶解在悬浮介质中，并与无蛋白质的情况相比进行溶血测试。在储存期间发现溶血受到很大抑制（G≈A＞F），但只有G对剪切提供了显著的保护作用。
