Offeman R D, Williams M C
Biomater Med Devices Artif Organs. 1976;4(1):49-79. doi: 10.3109/10731197609118644.
Rotating disks were used to hemolyze blood under low-stress laminar flow conditions. In the first sequence of tests, kinetic hemolysis curves (KHC) were obtained with polyethylene disks for three well-characterized bloods and repeated over a period of four weeks. Each blood had a KHC with different shape, which maintained its characteristics while aging. Correlations were sought between D6000 (percent of complete hemolysis, after 6000 sec of shear) and D0 (measured before shear) by two means of data analysis, in terms of blood chemistry. It was found that uric acid and very-low-density lipoprotein levels were most useful in predicting the characteristic D6000 vs. D0 relation for each blood, and that glucose levels correlated the rate of aging as measured by hemolysis. Other chemical factors are also displayed in terms of their influence on D0. The second series of tests consisted of comparing the KHC for four disk materials using a fourth blood, then repeating with a fifth blood. Hemolytic rankings of the materials were the same with these two blood, although the KHC shapes differed. The rankings were: polyvinyl chloride greater than Silastic approximately equal to polyethylene greater than polyether urethane, with PVC most hemolytic. In another sequence for examining materials effects, five different bloods were used to compare the hemolytic properties of Teflon, nylon, and polyethylene disks. Although the KHC for the three disks bore different relationships to each other with each different blood, extrapolation of data beyond 6000 sec suggests a ranking of Teflon greater than nylon greater than polyethylene.
在低应力层流条件下，使用旋转圆盘使血液发生溶血。在第一组测试中，使用聚乙烯圆盘对三种特征明确的血液获取了动态溶血曲线（KHC），并在四周的时间内重复进行。每种血液都有一条形状不同的KHC，其在老化过程中保持自身特征。通过两种数据分析方法，从血液化学角度寻求D6000（剪切6000秒后完全溶血的百分比）与D0（剪切前测量值）之间的相关性。结果发现，尿酸和极低密度脂蛋白水平在预测每种血液的特征性D6000与D0关系方面最为有用，并且葡萄糖水平与通过溶血测量的老化速率相关。还展示了其他化学因素对D0的影响。第二组测试包括使用第四种血液比较四种圆盘材料的KHC，然后用第五种血液重复进行。尽管KHC形状不同，但这两种血液对材料的溶血排名相同。排名为：聚氯乙烯大于硅橡胶近似等于聚乙烯大于聚醚聚氨酯，其中聚氯乙烯的溶血作用最强。在另一组研究材料效应的实验中，使用五种不同的血液比较聚四氟乙烯、尼龙和聚乙烯圆盘的溶血特性。尽管三种圆盘的KHC在每种不同血液中相互之间呈现不同关系，但超过6000秒的数据外推表明排名为聚四氟乙烯大于尼龙大于聚乙烯。
