Vember V V, Zhdanova N N
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiol Z. 2001 May-Jun;63(3):3-12.
Results on determination of radial (linear) growth rate of melanin-containing fungi Cladosporium sphaerospermum Penz. and Alternaria alternata (Fr.) Keissler concerning place of their isolation and ecologic peculiarities are presented in the paper. Essential differences, as to all studied indices of the strains of C. sphaerospermum strains, isolated from premices of the ChNPP 4th unit from the control strains of this species have been established. Only strain 34 of C. sphaerospermum, isolated from the cable driving region of the "Shelter" object, which did not differ from the control strains as to all the studied indices, made the exception. The radial growth rate was 2-4 times as low and variability amplitude 10-30 times as high in strains 60 and 5-1 of C. sphaerospermum, (which suffered high radiation load), as in the control strains of this species. The radial growth rate of C. sphaerospermum studied strains was an order lower as a whole than that of A. alternata strains. All the above said evidence for implementation of K-type vital strategy by C. sphaerospermum species and in this connection one can suppose its active growth on the surface of walls and ferroconcrete structures of the ChNPP 4th unit. Absence of differences, as to the studied radial growth indices, between A. alternata strains isolated from the ChNPP 4th unit premices and control strains of the species can evidence for the existence of A. alternata species under the conditions of high radioactivity in the surviving state.
本文给出了关于含黑色素真菌球形枝孢(Cladosporium sphaerospermum Penz.)和链格孢(Alternaria alternata (Fr.) Keissler)的径向(线性)生长速率与其分离地点和生态特性关系的研究结果。已确定,从第4机组切尔诺贝利核电站厂房内分离出的球形枝孢菌株与该物种的对照菌株相比,在所有研究指标上存在显著差异。只有从“掩体”设施电缆驱动区域分离出的球形枝孢34号菌株是个例外,该菌株在所有研究指标上与对照菌株没有差异。与该物种的对照菌株相比,遭受高辐射剂量的球形枝孢60号和5 - 1号菌株的径向生长速率低2 - 4倍,变异幅度高10 - 30倍。总体而言,所研究的球形枝孢菌株的径向生长速率比链格孢菌株低一个数量级。上述所有情况表明球形枝孢物种实施了K型生存策略,据此可以推测它在第4机组切尔诺贝利核电站厂房墙壁和钢筋混凝土结构表面活跃生长。从第4机组切尔诺贝利核电站厂房内分离出的链格孢菌株与该物种的对照菌株相比,在所研究的径向生长指标上没有差异,这可以证明链格孢物种在高放射性条件下以存活状态存在。