Buku A, Price J A, Mendlowitz M, Masur S
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA.
Peptides. 2001 Dec;22(12):1993-8. doi: 10.1016/s0196-9781(01)00542-3.
Fluorescent and biotinylated analogs of mast cell degranulating (MCD) peptide were synthesized and the labels fluoresceinisothiocyanate and N-hydroxysuccinimidobiotin were conjugated at position 1 in the MCD peptide sequence. The analogs with these moieties retained histamine-releasing activity as high as that of the parent MCD peptide in rat peritoneal mast cell assays. These labeled analogs were used in rat basophilic leukemia cells (RBL-2H3) to demonstrate by confocal microscopy and flow cytometry the specific binding of MCD peptide to mast cell receptors. Consequently MCD peptide was found to compete with and inhibit the binding of fluorescent IgE on RBL cells as monitored by flow cytometry. Thus MCD peptide may prove to be useful in the study of IgE receptor-bearing cells.
合成了肥大细胞脱颗粒（MCD）肽的荧光和生物素化类似物，并将异硫氰酸荧光素和N-羟基琥珀酰亚胺生物素标记物缀合在MCD肽序列的第1位。在大鼠腹膜肥大细胞试验中，带有这些部分的类似物保留了与母体MCD肽一样高的组胺释放活性。这些标记的类似物用于大鼠嗜碱性白血病细胞（RBL-2H3），通过共聚焦显微镜和流式细胞术证明MCD肽与肥大细胞受体的特异性结合。因此，通过流式细胞术监测发现，MCD肽可竞争并抑制荧光IgE在RBL细胞上的结合。因此，MCD肽可能在研究携带IgE受体的细胞方面有用。
