Hiller Reinhard, Laffer Sylvia, Harwanegg Christian, Huber Martin, Schmidt Wolfgang M, Twardosz Anna, Barletta Bianca, Becker Wolf M, Blaser Kurt, Breiteneder Heimo, Chapman Martin, Crameri Reto, Duchêne Michael, Ferreira Fatima, Fiebig Helmut, Hoffmann-Sommergruber Karin, King Te Piao, Kleber-Janke Tamara, Kurup Viswanath P, Lehrer Samuel B, Lidholm Jonas, Müller Ulrich, Pini Carlo, Reese Gerald, Scheiner Otto, Scheynius Annika, Shen Horng-Der, Spitzauer Susanne, Suck Roland, Swoboda Ines, Thomas Wayne, Tinghino Raffaela, Van Hage-Hamsten Marianne, Virtanen Tuomas, Kraft Dietrich, Müller Manfred W, Valenta Rudolf
VBC-GENOMICS, A-1030 Vienna, Austria.
FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14.
Type I allergy is an immunoglobulin E (IgE)-mediated hypersensitivity disease affecting more than 25% of the population. Currently, diagnosis of allergy is performed by provocation testing and IgE serology using allergen extracts. This process defines allergen-containing sources but cannot identify the disease-eliciting allergenic molecules. We have applied microarray technology to develop a miniaturized allergy test containing 94 purified allergen molecules that represent the most common allergen sources. The allergen microarray allows the determination and monitoring of allergic patients' IgE reactivity profiles to large numbers of disease-causing allergens by using single measurements and minute amounts of serum. This method may change established practice in allergy diagnosis, prevention, and therapy. In addition, microarrayed antigens may be applied to the diagnosis of autoimmune and infectious diseases.
I型过敏是一种由免疫球蛋白E(IgE)介导的超敏反应疾病,影响着超过25%的人口。目前,过敏的诊断是通过激发试验和使用过敏原提取物的IgE血清学检测来进行的。这个过程确定了含有过敏原的来源,但无法识别引发疾病的过敏原分子。我们应用微阵列技术开发了一种小型化的过敏测试,其中包含94种纯化的过敏原分子,这些分子代表了最常见的过敏原来源。过敏原微阵列能够通过单次测量和微量血清来测定和监测过敏患者对大量致病过敏原的IgE反应谱。这种方法可能会改变过敏诊断、预防和治疗的既定做法。此外,微阵列抗原可应用于自身免疫性疾病和感染性疾病的诊断。