Evans S A, Adams R, Rainger G E
School of Applied Sciences, University of Wales Institute Cardiff, Western Avenue, Cardiff CF5 2YB, UK.
Clin Hemorheol Microcirc. 2001;25(2):63-73.
The effect of increased adhesiveness and decreased deformability of leukocytes following activation can have a profound effect on flow through the microcirculation. Measurement of leukocyte deformability is therefore an important tool in the study of the pathology of vascular diseases. Although much work has been done on the rheological properties of lymphocytes and granulocytes, there is little information available on the larger mononuclear cells, the monocytes. To investigate monocyte rheology, attempts were made to purify monocytes by a variety of methods. Purified monocytes were then filtered through 5 microm polycarbonate filters, with flow profiles (change in volume with time) recorded over 300 seconds. The profiles were analysed by least squares fitting to an appropriate mathematical model. Analysis of filtration data demonstrated 3 distinct sub-populations of monocytes with differing rheological properties. Other workers have characterised monocytes into defined subsets on the basis of their size, phagocytic ability or expression of cell surface markers. The definition of monocytes into defined rheological subsets is a new and useful addition to these studies.
白细胞激活后黏附性增加和变形性降低，这会对微循环血流产生深远影响。因此，测量白细胞变形性是研究血管疾病病理学的一项重要工具。尽管在淋巴细胞和粒细胞的流变学特性方面已经开展了大量工作，但关于较大的单核细胞，即单核细胞的相关信息却很少。为了研究单核细胞流变学，人们尝试通过多种方法纯化单核细胞。然后将纯化的单核细胞通过5微米的聚碳酸酯滤膜进行过滤，并记录300秒内的流量曲线（体积随时间的变化）。通过最小二乘法拟合到合适的数学模型对这些曲线进行分析。过滤数据分析显示，单核细胞存在3个具有不同流变学特性的不同亚群。其他研究人员已根据单核细胞的大小、吞噬能力或细胞表面标志物的表达将其分为不同的亚群。将单核细胞定义为具有明确流变学特征的亚群是这些研究中一项新的且有用的补充。
