Orea J M, Montero C, Jiménez J B, Ureña A G
Unidad de Láseres y Haces Moleculares, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Spain.
Anal Chem. 2001 Dec 15;73(24):5921-9. doi: 10.1021/ac010439p.
A method has been developed for nonvolatile compound analysis in fruits, namely, trans-resveratrol in grapes and vine leaves. This has been accomplished by the combination of laser desorption with resonance-enhanced multiphoton ionization coupled to time-of-flight mass spectrometry detection. After the optimization of the experimental conditions and the finding of the resonant wave-length of the substance, the full validation of the technique (i.e., linearity, repeatability, reproducibility, accuracy, detection limit, and quantification limit) was carried out with satisfactory results. Essential features of the method are as follows: (1) an enhanced desorption yield due to the mixing of the analyte with metal powder and (2) a high resolution and sensitivity and a low detection limit due to laser resonant ionization and mass spectrometric detection. Application to the analysis of trans-resveratrol in vine leaves and grape skin demonstrated the capabilities of the analytical method reaching detection limits of only few ppb.
已开发出一种用于水果中非挥发性化合物分析的方法，即用于分析葡萄和葡萄叶中的反式白藜芦醇。该方法是通过将激光解吸与共振增强多光子电离相结合，并与飞行时间质谱检测联用实现的。在优化实验条件并找到该物质的共振波长后，对该技术进行了全面验证（即线性、重复性、再现性、准确性、检测限和定量限），结果令人满意。该方法的主要特点如下：（1）由于分析物与金属粉末混合，解吸产率提高；（2）由于激光共振电离和质谱检测，具有高分辨率、高灵敏度和低检测限。将该方法应用于葡萄叶和葡萄皮中反式白藜芦醇的分析，结果表明该分析方法的检测限仅为几ppb。
