Jean-Denis J Bernard, Pezet Roger, Tabacchi Raffaele
Institute of Chemistry, University of Neuchatel, Rue Emile Argand 11, CH-2009 Neuchatel, Switzerland.
J Chromatogr A. 2006 Apr 21;1112(1-2):263-8. doi: 10.1016/j.chroma.2006.01.060. Epub 2006 Feb 3.
Resveratrol, trans-epsilon-viniferin and trans-delta-viniferin are the major stilbenes induced in downy mildew infected grapevine leaves. In addition, nine minor polyphenolic compounds, described as stilbenes derivatives, have been separated and detected among known stilbenes after a methanolic microextraction of small pieces (1-2 mg) from infected grapevine leaves with a rapid, qualitative and optimized HPLC method coupled to mass spectrometry using atmospheric pressure photoionisation (APPI-MS(n)). The characterization of unknown stilbenic derivatives as six resveratrol dimers, two dimethylated resveratrol dimers and a resveratrol trimer are reported. Therefore, structures have been proposed for the dimethylated resveratrol dimers. Use of an easy sample treatment and the LC-APPI-MS(n) method results in spectral data of these minor naturally occurring viniferin analogues.
白藜芦醇、反式-ε-葡萄素和反式-δ-葡萄素是霜霉病感染的葡萄叶片中诱导产生的主要芪类化合物。此外，在用一种快速、定性且优化的高效液相色谱法（HPLC）与大气压光电离质谱联用（APPI-MS(n)）从小片（1-2毫克）感染葡萄叶片进行甲醇微萃取后，在已知芪类化合物中分离并检测到了九种被描述为芪类衍生物的次要多酚化合物。报道了将未知芪类衍生物鉴定为六种白藜芦醇二聚体、两种二甲基化白藜芦醇二聚体和一种白藜芦醇三聚体。因此，已提出二甲基化白藜芦醇二聚体的结构。使用简便的样品处理方法和LC-APPI-MS(n)方法得到了这些次要天然存在的葡萄素类似物的光谱数据。
