Gamini Amelia, Paoletti Sergio, Toffanin Renato, Micali Fulvio, Michielin Luigi, Bevilacqua Carla
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy.
Biomaterials. 2002 Feb;23(4):1161-7. doi: 10.1016/s0142-9612(01)00231-9.
Structural properties of several cross-linked hyaluronan derivatives, obtained by scanning electron microscopy, monodimensional NMR microscopy and small angle X-ray scattering of synchrotron radiation, are presented and compared with those observed for non-modified hyaluronic acid, used as a reference material. The experimental results, obtained in different media, showed a consistent picture of the synthesized matrices. In particular, the presence of zones of denser polymeric material observed by electron microscopy resulted in a higher transversal relaxation rate of the bulk water protons as well as in a decrease of the diffusion coefficient obtained by NMR microscopy. Moreover, the presence of polymer junction zones gave rise to the appearance of a well-defined correlation peak in the pattern of intensity of the scattered X-radiation.
通过扫描电子显微镜、一维核磁共振显微镜以及同步辐射小角X射线散射获得了几种交联透明质酸衍生物的结构特性，并将其与用作参考材料的未改性透明质酸的结构特性进行了比较。在不同介质中获得的实验结果显示了合成基质的一致情况。特别是，电子显微镜观察到存在较致密聚合物材料区域，这导致大量水质子的横向弛豫率更高，以及核磁共振显微镜测得的扩散系数降低。此外，聚合物连接区的存在导致在散射X射线强度模式中出现一个明确的相关峰。
