School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA.
Acta Biomater. 2010 Jul;6(7):2407-14. doi: 10.1016/j.actbio.2009.12.049. Epub 2010 Jan 4.
Development of biomaterials that provide mechanical and molecular cues for wound healing and regeneration must meet several design parameters. In addition to high biocompatibility, biomaterials should possess suitable porosity as well as the ability to be chemically tailored to control parameters including biodegradability and bioactivity. These characteristics were studied in hyaluronan (HA), a natural polymer found in the body. HA was modified with thiol cross-linking sites to form a stable hydrogel scaffold to examine effects in in vitro cortical cell growth. HA with 20% and 44% thiolation was used to make gels at 0.5%, 0.75%, 1%, and 1.25% (w/v). Results indicate that the bioactivity of the HA after functionalization, as determined by degree of substitution (HA thiolation), has a greater effect on neurite outgrowth than does gel stiffness. The lower substituted HA (20%) promoted greater neurite growth as compared to the higher substituted HA (44%).
开发能够为伤口愈合和再生提供机械和分子线索的生物材料必须满足几个设计参数。除了高生物相容性外，生物材料还应具有适当的孔隙率，并能够进行化学修饰以控制包括生物降解性和生物活性在内的参数。这些特性在体内存在的天然聚合物透明质酸 (HA) 中进行了研究。HA 用硫醇交联位点进行修饰以形成稳定的水凝胶支架，以研究体外皮质细胞生长的影响。用 20%和 44%硫醇化的 HA 制成 0.5%、0.75%、1%和 1.25%（w/v）的凝胶。结果表明，功能化后 HA 的生物活性（通过取代度确定）对神经突生长的影响大于凝胶硬度。与高取代的 HA（44%）相比，低取代的 HA（20%）促进了更大的神经突生长。
