Mukai M, Bohgaki T, Kondo M, Notoya A, Kohno M
Division of Clinical Immunology and Hematology, Department of Medicine, Sapporo City General Hospital, Kita 11-jo, Nishi 13-chome, Chuo-ku, Sapporo 060-8604, Japan.
Ann Hematol. 2001 Dec;80(12):715-21. doi: 10.1007/s00277-001-0382-y. Epub 2001 Oct 23.
The purpose of this study was to analyze changes in the T-helper cell 1/T-helper cell 2 (Th1/Th2) balance of peripheral T-helper cells after autologous peripheral blood stem cell transplantation (auto-PBSCT) for non-Hodgkin's lymphoma (NHL) and to evaluate the effects of chemotherapy and granulocyte colony-stimulating factor (G-CSF) on the Th1/Th2 balance. A series of peripheral blood samples from four patients with NHL were collected before peripheral blood stem cell harvest and after auto-PBSCT. Using flow cytometry, Th1 and Th2 cells were identified by their intracellular cytokines: interleukin (IL)-4-/interferon (IFN)-gamma+ and IL-4+/IFN-gamma-, respectively. The Th1/Th2 balance was estimated as the ratio of IL4-/IFN-gamma+ cells to IL-4+/IFN-gamma- cells. Although the Th1/Th2 balance decreased initially, it increased markedly 28 days after the cessation of G-CSF, following auto-PBSCT, in parallel with an increase in lymphocytes. This increase was mainly due to an increase in the proportion of Th1 cells. The Th1/Th2 balance did not change appreciably before PBSC harvest. Serum IFN-gamma increased after auto-PBSCT in three patients. These preliminary data demonstrate that, after auto-PBSCT for NHL, the Th1/Th2 balance decreases initially and then increases after 1 month to levels above pretreatment levels and that the effects of chemotherapy and G-CSF on the Th1/Th2 balance are negligible before PBSC harvest. Further evaluation of the Th1/Th2 balance after allogeneic PBSCT at the single-cell level should be undertaken using this method.
本研究的目的是分析非霍奇金淋巴瘤（NHL）患者自体外周血干细胞移植（auto-PBSCT）后外周辅助性T细胞的辅助性T细胞1/辅助性T细胞2（Th1/Th2）平衡变化，并评估化疗和粒细胞集落刺激因子（G-CSF）对Th1/Th2平衡的影响。收集了4例NHL患者在采集外周血干细胞前及auto-PBSCT后的一系列外周血样本。采用流式细胞术，通过细胞内细胞因子分别鉴定Th1和Th2细胞：白细胞介素（IL）-4-/干扰素（IFN）-γ+和IL-4+/IFN-γ-。Th1/Th2平衡以IL4-/IFN-γ+细胞与IL-4+/IFN-γ-细胞的比例来估算。尽管Th1/Th2平衡最初下降，但在auto-PBSCT后停用G-CSF 28天时显著增加，同时淋巴细胞增多。这种增加主要是由于Th1细胞比例增加。在采集外周血干细胞前，Th1/Th2平衡没有明显变化。3例患者在auto-PBSCT后血清IFN-γ升高。这些初步数据表明，NHL患者auto-PBSCT后，Th1/Th2平衡最初下降，1个月后升高至预处理水平以上，且在采集外周血干细胞前化疗和G-CSF对Th1/Th2平衡的影响可忽略不计。应使用该方法在单细胞水平上进一步评估异基因PBSCT后的Th1/Th2平衡。
