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一种新型狂犬病病毒表达盒表明，亲缘关系较远的莫科拉病毒能够拯救有缺陷的狂犬病病毒基因组。

A novel expression cassette of lyssavirus shows that the distantly related Mokola virus can rescue a defective rabies virus genome.

作者信息

Le Mercier Philippe, Jacob Yves, Tanner Kyle, Tordo Noël

机构信息

Laboratoire des Lyssavirus, Institut Pasteur, 75724 Paris Cédex 15, France.

出版信息

J Virol. 2002 Feb;76(4):2024-7. doi: 10.1128/jvi.76.4.2024-2027.2002.

DOI:10.1128/jvi.76.4.2024-2027.2002
PMID:11799201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC135871/
Abstract

By comparing three expression vectors for the rabies virus (Rv) minigenome, we show that the characteristic of the Rv RNA is important for efficient rescue despite its not being crucial for replication. Moreover, we show that the coexpression of the viral proteins from helper Rv and Mokola virus could rescue the Rv minigenome while Rv-related European bat lyssavirus 1 could not, suggesting that the signals controlling transcription and replication are conserved in the distantly related Rv and Mokola virus.

摘要

通过比较三种用于狂犬病病毒（Rv）微型基因组的表达载体，我们发现Rv RNA的特性对于有效拯救至关重要，尽管它对复制并非至关重要。此外，我们表明来自辅助Rv和莫科拉病毒的病毒蛋白共表达可以拯救Rv微型基因组，而与Rv相关的欧洲蝙蝠狂犬病病毒1则不能，这表明控制转录和复制的信号在远缘相关的Rv和莫科拉病毒中是保守的。

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