Bourhy H, Kissi B, Tordo N
Unité de la Rage, Institut Pasteur, Paris, France.
Virology. 1993 May;194(1):70-81. doi: 10.1006/viro.1993.1236.
The sequence of 5568 nucleotides of the 3' moiety of the Mokola virus genome (serotype 3 of lyssaviruses) encompassing the nucleoprotein (N), phosphoprotein, matrix protein, and glycoprotein genes is presented and compared to that of the vaccinal strains of serotype 1. It allowed us to determine consensus sequences derived from the transcriptional start/stop signals and the order of protein conservation (nucleoprotein > matrix protein > phosphoprotein) in lyssaviruses. The sequences of the N gene of a fox rabies virus isolate from France (serotype 1), Lagos bat virus (serotype 2), Duvenhage virus (serotype 4), two European bat lyssaviruses (EBL) subtype 1, and two EBL subtype 2 were also determined to study the genetic diversity throughout the whole Lyssavirus genus and reinvestigate the classification of this genus. Six clearly distinct genotypes can be distinguished according to their percentage of amino acid similarity. Genotypes 2 (Lagos bat virus) and 3 (Mokola virus) are the most phylogenetically distant from the vaccinal and classical rabies viruses of genotype 1. Genotypes 4 (Duvenhage virus) and 5 (EBL1) are closely related to each other. Genotype 6 is represented by EBL2. Compared to the N proteins of the four principal serotypes of the Vesiculovirus genus (vesicular stomatitis virus serotype New Jersey and serotype Indiana, Chandipura virus, and Piry virus), the N gene of lyssaviruses exhibits a lower genetic variability.
本文呈现了莫科拉病毒基因组（狂犬病毒属血清型3）3'部分包含核蛋白（N）、磷蛋白、基质蛋白和糖蛋白基因的5568个核苷酸序列，并将其与血清型1的疫苗株序列进行了比较。这使我们能够确定源自转录起始/终止信号的共有序列以及狂犬病毒属中蛋白质保守性的顺序（核蛋白>基质蛋白>磷蛋白）。还测定了来自法国的狐狂犬病病毒分离株（血清型1）、拉各斯蝙蝠病毒（血清型2）、杜文哈格病毒（血清型4）、两种欧洲蝙蝠狂犬病毒（EBL）亚型1和两种EBL亚型2的N基因序列，以研究整个狂犬病毒属的遗传多样性并重新调查该属的分类。根据氨基酸相似百分比可区分出六种明显不同的基因型。基因型2（拉各斯蝙蝠病毒）和3（莫科拉病毒）在系统发育上与基因型1的疫苗株和经典狂犬病病毒距离最远。基因型4（杜文哈格病毒）和5（EBL1）彼此密切相关。基因型6由EBL2代表。与水疱性口炎病毒属的四种主要血清型（新泽西血清型和印第安纳血清型水疱性口炎病毒、钱迪普拉病毒和皮里病毒）的N蛋白相比，狂犬病毒属的N基因表现出较低的遗传变异性。
