Purcell W M, Cohen D L, Hanahoe T H
Hatfield Polytechnic, UK.
Int Arch Allergy Appl Immunol. 1989;90(4):382-6. doi: 10.1159/000235058.
Rat mast cells, isolated from different anatomical locations, were found to exhibit heterogeneity with respect to histamine and 5-hydroxytryptamine (5-HT) content. Unlike peritoneal mast cells, those derived by enzymatic digestion of rat lung and mesentery tissues contained amounts of 5-HT in excess of histamine. Both compound 48/80 and A23187 were more effective liberators of histamine than 5-HT in each cell type, despite their differences in relative amine content. However, in response to a mixture of specific antigen and phosphatidylserine, the pattern of secretion of both amines from each mast cell type was observed to be essentially colinear. These results are discussed in terms of amine release mechanisms and post-secretory events.
从不同解剖部位分离出的大鼠肥大细胞，在组胺和5-羟色胺（5-HT）含量方面表现出异质性。与腹膜肥大细胞不同，通过酶消化大鼠肺和肠系膜组织获得的肥大细胞所含的5-HT量超过组胺。尽管每种细胞类型中相对胺含量存在差异，但化合物48/80和A23187在释放组胺方面比释放5-HT更有效。然而，在对特异性抗原和磷脂酰丝氨酸混合物的反应中，观察到每种肥大细胞类型释放这两种胺的模式基本呈共线性。从胺释放机制和分泌后事件的角度对这些结果进行了讨论。
