Purcell W M, Hanahoe T H
Hatfield Polytechnic, Herts, UK.
Agents Actions. 1990 Apr;30(1-2):38-40. doi: 10.1007/BF01968992.
Despite the fact that mast cells isolated from rat mesentery and lung tissue contain 5-HT in excess of histamine, the pattern of amine output, in response to compound 48/80, mirrors that in peritoneal mast cells where histamine is in excess of 5-HT, such that this secretagogue induces a greater percentage release of histamine than 5-HT. Mast cells are capable of taking up both these amines, although uptake of 5-HT is in preference to that of histamine. With concentrations of clomipramine and fluoxetine that inhibited 5-HT uptake, the net percentage release of 5-HT increased in a corresponding manner, and the concentration-effect curves in response to compound 48/80 ran in a collinear fashion with that of control histamine output (measured in the absence of the drugs). Both drugs had no effect upon the uptake or secretion of histamine. Thus, observed differences in the apparent pattern of histamine and 5-HT secretion from rat mast cells may be due to selective reuptake of amines rather than a reflection of differential amine release.
尽管从大鼠肠系膜和肺组织分离出的肥大细胞所含5-羟色胺（5-HT）超过组胺，但对化合物48/80的胺释放模式，与组胺含量超过5-HT的腹膜肥大细胞中的模式相似，即这种促分泌剂诱导组胺的释放百分比高于5-HT。肥大细胞能够摄取这两种胺，尽管对5-HT的摄取优先于组胺。使用抑制5-HT摄取的氯米帕明和氟西汀浓度时，5-HT的净释放百分比相应增加，并且对化合物48/80的浓度-效应曲线与对照组胺释放（在无药物情况下测量）呈共线关系。两种药物对组胺的摄取或分泌均无影响。因此，观察到的大鼠肥大细胞组胺和5-HT分泌明显模式的差异可能是由于胺的选择性再摄取，而非胺释放差异的反映。
