Beccari Simone, Teixeira Luís, Rørth Pernille
Developmental Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
Mech Dev. 2002 Feb;111(1-2):115-23. doi: 10.1016/s0925-4773(01)00615-3.
During Drosophila oogenesis, border cells perform a stereotypic migration. Slbo, a C/EBP transcription factor, is required for this migration. Here we identify Drosophila Stat92E (signal transducer and activator of transcription) in a screen for gain-of-function suppressors of the slbo mutant phenotype. By clonal analysis for Stat92E and hop (Drosophila JAK) mutants we find that the JAK/STAT pathway is required in border cells for their migration. The activating ligand for the pathway, Unpaired, is expressed in polar cells. Polar cells are specialized cells which can induce border cell fate in anterior follicle cells. On its own, ectopic expression of Unpaired can induce ectopic expression of border cell markers including Slbo. However, Stat92E mutant cells still express normal levels of Slbo protein, thus Stat92E must regulate other targets critical for border cell migration.
在果蝇卵子发生过程中，边界细胞进行一种模式化迁移。Slbo作为一种C/EBP转录因子，是这种迁移所必需的。在此，我们在筛选slbo突变体表型的功能获得性抑制子时鉴定出了果蝇Stat92E（信号转导子和转录激活子）。通过对Stat92E和hop（果蝇JAK）突变体进行克隆分析，我们发现边界细胞迁移需要JAK/STAT信号通路。该信号通路的激活配体Unpaired在极性细胞中表达。极性细胞是能够诱导前卵泡细胞产生边界细胞命运的特化细胞。单独而言，异位表达Unpaired可诱导包括Slbo在内的边界细胞标志物的异位表达。然而，Stat92E突变细胞仍表达正常水平的Slbo蛋白，因此Stat92E必定调控对边界细胞迁移至关重要的其他靶标。
