Rørth P, Szabo K, Texido G
Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Mol Cell. 2000 Jul;6(1):23-30. doi: 10.1016/s1097-2765(05)00008-0.
The C/EBP transcription factor, Slbo, is required for migration of border cells during Drosophila oogenesis. Unexpectedly, we find that neither increase nor decrease of Slbo activity is tolerated in border cells. Correct protein level is in part ensured by cell type-specific regulated turnover of Slbo protein. Through genetic screening, we identify two genes that are involved in this regulation. The Ubp64 ubiquitin hydrolase acts as a stabilizer of Slbo protein. A novel gene, tribbles, is a negative regulator of slbo in vivo. Tribbles acts by specifically targeting Slbo for rapid degradation via ubiquitination.
C/EBP转录因子Slbo是果蝇卵子发生过程中边缘细胞迁移所必需的。出乎意料的是，我们发现在边缘细胞中，Slbo活性的增加或降低都无法被容忍。Slbo蛋白细胞类型特异性的调节性周转在一定程度上确保了其正确的蛋白水平。通过基因筛选，我们鉴定出两个参与这种调节的基因。泛素水解酶Ubp64作为Slbo蛋白的稳定剂。一个新基因tribbles是体内Slbo的负调节因子。Tribbles通过特异性地将Slbo靶向泛素化以进行快速降解来发挥作用。
