Oksonen J, Sorokin L M, Hormia M
University of Turku, Institute of Dentistry, Finland.
J Dent Res. 2001 Dec;80(12):2093-7. doi: 10.1177/00220345010800121401.
It is not known whether epithelial differentiation patterns are reflected in the composition of gingival basement membranes (BMs). We have investigated the expression of laminin isoforms and associated BM components in the murine dento-epithelial junction by using immunofluorescence microscopy. Our results show that chains of laminins 5/6/7/10/11 are expressed in the BM of outer gingival epithelium. The external BM between junctional epithelium (JE) and connective tissue differs from gingival BM by lacking laminin-7 and -11 chains. The internal basal lamina (IBL) between JE and tooth contains only laminin-5. Collagen chains alpha1,2(IV) and nidogen-1 are present in other BMs except the IBL. The dento-epithelial junction thus has a unique BM composition, suggesting that epithelial cells are able to secrete two extracellular matrices in a polarized manner. The exclusive expression of the non-self-polymerizing laminin-5 indicates that the IBL is not a BM by definition, but rather a simple extracellular matrix lacking network structure.
目前尚不清楚上皮分化模式是否反映在牙龈基底膜（BMs）的组成中。我们通过免疫荧光显微镜研究了层粘连蛋白异构体和相关BM成分在小鼠牙上皮交界处的表达。我们的结果表明，层粘连蛋白5/6/7/10/11链在外牙龈上皮的BM中表达。结合上皮（JE）和结缔组织之间的外部BM与牙龈BM不同，缺乏层粘连蛋白-7和-11链。JE和牙齿之间的内部基膜（IBL）仅包含层粘连蛋白-5。除IBL外，其他BM中存在胶原链α1,2(IV)和巢蛋白-1。因此，牙上皮交界处具有独特的BM组成，这表明上皮细胞能够以极化方式分泌两种细胞外基质。非自聚合层粘连蛋白-5的唯一表达表明，IBL根据定义不是BM，而是缺乏网络结构的简单细胞外基质。
