Guo S P, Wang W L, Zhai Y Q, Zhao Y L
Department of Pathology, Xijing Hospital of the Fourth Military Medical University, Xi'an, China.
World J Gastroenterol. 2001 Jun;7(3):340-4. doi: 10.3748/wjg.v7.i3.340.
In this study we investigated the relationship of the X protein of HBV and nuclear factor-kappa B (NF-kappa B) and the expression of NF-kappa B in human hepatocellular carcinoma tissues.
Immunohistochemistry SP method was used to detect the expression of NF-kappa B and the X protein of HBV in human hepatocellular carcinoma tissues of 52 cases. Gene transfection mediated by lipofectamine was used to transfect the eukaryotic expression vector pCDNA3.1-HBX of HBV x gene into human hepatocellular carcinoma cell line HCC-9204 and NF-kappa B was detected.
NF kappa B was widely expressed in human hepatocellular carcinoma tissues in a total of 52 cases and its expression was related to the X protein of HBV. NF-kappa B was localized both in the cytoplasm and the nuclei of hepatocellular carcinoma cells in 11 cases which were positive for the X protein of HBV while in 41 cases negative for the X protein of HBV, NF-kappa B was only localized in the cytoplasm of hepatocellular carcinoma cells but translocated to the nuclei of hepatocellular carcinoma cells after the eukaryotic expression vector pCDNA3.1-HBX was transfected into HCC-9204 cells.
This study strongly suggests that the nuclear factor NF-kappa B is widely expressed in hepatocellular carcinoma tissues in different styles according to the expression of the X protein of HBV. NF-kappa B is abnormally activated in hepatocellular carcinoma, which is probably related to the X protein of HBV. The X protein of HBV can activate NF-kappa B to translocate into nuclei of hepatocellular carcinoma cells.
本研究探讨乙型肝炎病毒（HBV）X蛋白与核因子-κB（NF-κB）的关系以及NF-κB在人肝细胞癌组织中的表达情况。
采用免疫组织化学SP法检测52例人肝细胞癌组织中NF-κB及HBV X蛋白的表达。利用脂质体介导的基因转染技术将HBV x基因的真核表达载体pCDNA3.1-HBX转染至人肝癌细胞系HCC-9204，并检测NF-κB。
52例人肝细胞癌组织中NF-κB均有广泛表达，其表达与HBV X蛋白有关。11例HBV X蛋白阳性的肝癌细胞中，NF-κB定位于细胞质和细胞核；41例HBV X蛋白阴性的肝癌细胞中，NF-κB仅定位于细胞质，但将真核表达载体pCDNA3.1-HBX转染至HCC-9204细胞后，NF-κB转位至细胞核。
本研究强烈提示，核因子NF-κB在肝细胞癌组织中的表达随HBV X蛋白表达情况呈现不同模式。NF-κB在肝细胞癌中异常激活，这可能与HBV X蛋白有关。HBV X蛋白可激活NF-κB转位至肝癌细胞核。
