中国人群中自身免疫性肝炎与HLA - DR4及第三高变区DRβ等位基因序列的关系
Relationship between autoimmune hepatitis and HLA-DR4 and DRbeta allelic sequences in the third hypervariable region in Chinese.
作者信息
Ma X, Qiu D K
机构信息
Shanghai Institute of Digestive Disease, Shanghai Second Medical University, Affiliated Renji Hospital, 145 Shandong Zhonglu, Shanghai 200001, China.
出版信息
World J Gastroenterol. 2001 Oct;7(5):718-21. doi: 10.3748/wjg.v7.i5.718.
AIM
To analyze the association of HLA-DRB1 with autoimmune hepatitis (AIH) in patients from China.
METHODS
In 32 patients and 48 healthy controls, polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 with AIH.
RESULTS
HLA-DRB1 typing by PCR-SSP showed that DR4 had a significantly increased frequency among patients with AIH versus healthy control (46.9% versus 20.8%; relative risk = 3.35, P=0.014). In subtypes of DR4, there was a trend of increase in the gene frequency of DRB1 0405 in patients with AIH versus healthy controls (21.9% vs 6.3%, P=0.04, but P(c) =0.08). In addition, a significant increase was found in the alleles frequency encoding QRRAA from the third hyperpolymorphic region of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P=0.016, P(c)=0.028, RR=9.75).
CONCLUSION
AIH in Chinese is associated with HLA-DR4. There is a relationship between QRRAA sequence within the third hyperpolymorphic region of the DRB allele and AIH in Chinese.
目的
分析在中国患者中人类白细胞抗原-DRB1（HLA-DRB1）与自身免疫性肝炎（AIH）的相关性。
方法
对32例患者和48例健康对照者，采用序列特异性引物聚合酶链反应扩增（PCR-SSP）检测HLA-DRB1的某些等位基因或多态性序列与AIH的相关性。
结果
通过PCR-SSP进行的HLA-DRB1分型显示，与健康对照相比，AIH患者中DR4的频率显著增加（46.9%对20.8%；相对风险=3.35，P=0.014）。在DR4亚型中，与健康对照相比，AIH患者中DRB1 0405的基因频率有增加趋势（21.9%对6.3%，P=0.04，但校正P值=0.08）。此外，AIH患者中DR4第三个高多态性区域编码QRRAA的等位基因频率显著增加（DR4阳性患者中为86.7%，DR4阳性对照中为40.0%，P=0.016，校正P值=0.028，相对风险=9.75）。
结论
中国人群中的AIH与HLA-DR4相关。DRB等位基因第三个高多态性区域内的QRRAA序列与中国人群中的AIH存在关联。
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