The pediatrics department, Faculty of medicine, Ain Shams University Cairo, Egypt.
Ital J Pediatr. 2010 Nov 10;36:73. doi: 10.1186/1824-7288-36-73.
The human leukocyte antigens (HLAs) are proteins found in the membranes of nearly all nucleated cells. People with certain HLA antigens are more likely to develop certain autoimmune diseases. The aim of this study was to determine the frequency of HLA-DRB1 in children with autoimmune hepatitis (AIH) as a risk factor for occurrence, its relation to preceding hepatitis A infection and treatment outcome.
25 children with AIH were subjected to HLA-DRB 1 typing performed by sequence specific oligonucleotide probe technique and compared to HLA-DRB1 found in 548 normal populations.
The most frequent alleles found in our children with AIH were HLA-DRB113 (36%), HLA-DRB104 (18%) and HLA-DRB103 (14%). HLA-DRB113 was significantly more frequent in AIH patients compared to controls. In type I AIH patients HLA-DRB113 was the most frequent allele (32.4%), followed by HLA-DRB104 in (20.6%) and HLA-DRB103 in (14.7%), While in type II, the most frequent alleles were HLA-DRB113 in (40%), HLA-DRB107 (20%) and HLA-DRB115 in (20%). HLA-DRB1*12 was significantly more frequent in AIH patients with positive Hepatitis A IgM than in patients with negative hepatitis A IgM. No statistically significant difference between partial responders and complete responders to treatment as regards HLA-DRB1 subtypes.
It is concluded from the previous study that HLA-DRB113 may be a susceptibility allele for the occurrence of autoimmune hepatitis in our population. HLA-DRB107 and HLA-DRB115 may be susceptibility alleles for occurrence of autoimmune hepatitis type 2. HLA-DRB112 association with AIH in patients triggered by hepatitis A needs further studies.
人类白细胞抗原（HLAs）是存在于几乎所有有核细胞膜中的蛋白质。具有某些 HLA 抗原的人更容易患上某些自身免疫性疾病。本研究旨在确定人类白细胞抗原-DRB1 在自身免疫性肝炎（AIH）儿童中的频率作为发病的危险因素，及其与先前甲型肝炎感染和治疗结果的关系。
对 25 例 AIH 患儿进行 HLA-DRB1 分型，采用序列特异性寡核苷酸探针技术，并与 548 例正常人群的 HLA-DRB1 进行比较。
在我们的 AIH 患儿中发现的最常见等位基因是 HLA-DRB113（36%）、HLA-DRB104（18%）和 HLA-DRB103（14%）。与对照组相比，HLA-DRB113 在 AIH 患者中明显更为常见。在 I 型 AIH 患者中，HLA-DRB113 是最常见的等位基因（32.4%），其次是 HLA-DRB104（20.6%）和 HLA-DRB103（14.7%）；而在 II 型中，最常见的等位基因是 HLA-DRB113（40%）、HLA-DRB107（20%）和 HLA-DRB115（20%）。与甲型肝炎 IgM 阴性的 AIH 患者相比，甲型肝炎 IgM 阳性的 AIH 患者中 HLA-DRB1*12 明显更为常见。在治疗的部分和完全应答者之间，关于 HLA-DRB1 亚型没有统计学上的显著差异。
从之前的研究中得出结论，HLA-DRB113 可能是我们人群中发生自身免疫性肝炎的易感等位基因。HLA-DRB107 和 HLA-DRB115 可能是发生自身免疫性肝炎 2 型的易感等位基因。HLA-DRB112 与甲型肝炎触发的 AIH 的关联需要进一步研究。
