Serber Z, Richter C, Dötsch V
Graduate Group in Biophysics, University of California San Francisco, San Francisco, CA 94143 USA.
Chembiochem. 2001 Apr 2;2(4):247-51. doi: 10.1002/1439-7633(20010401)2:4<247::AID-CBIC247>3.0.CO;2-V.
We have started to develop new NMR pulse sequences that detect carbon magnetization during the acquisition period. These experiments have become possible with the recent introduction of cryogenic probe heads. We show that a careful design of these carbon-detected experiments can at least partially compensate for the inherent lower sensitivity of carbon detection compared to proton detection. We discuss potential applications of carbon detection and demonstrate a deconvolution technique that removes the effects of carbon-carbon couplings from the spectra.
我们已开始研发新的核磁共振脉冲序列，用于在采集期间检测碳磁化。随着近期低温探头的引入，这些实验得以实现。我们表明，对这些碳检测实验进行精心设计，至少可以部分弥补与质子检测相比碳检测固有的较低灵敏度。我们讨论了碳检测的潜在应用，并展示了一种去卷积技术，该技术可消除光谱中碳 - 碳耦合的影响。
