叶绿体基因工程的里程碑：生物技术领域的一个环境友好时代。
Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology.
作者信息
Daniell Henry, Khan Muhammad S, Allison Lori
机构信息
Dept of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32826-3227, USA.
出版信息
Trends Plant Sci. 2002 Feb;7(2):84-91. doi: 10.1016/s1360-1385(01)02193-8.
Abstract
Chloroplast genomes defied the laws of Mendelian inheritance at the dawn of plant genetics, and continue to defy the mainstream approach to biotechnology, leading the field in an environmentally friendly direction. Recent success in engineering the chloroplast genome for resistance to herbicides, insects, disease and drought, and for production of biopharmaceuticals, has opened the door to a new era in biotechnology. The successful engineering of tomato chromoplasts for high-level transgene expression in fruits, coupled to hyper-expression of vaccine antigens, and the use of plant-derived antibiotic-free selectable markers, augur well for oral delivery of edible vaccines and biopharmaceuticals that are currently beyond the reach of those who need them most.
摘要
在植物遗传学诞生之初，叶绿体基因组就违背了孟德尔遗传定律，并且持续挑战生物技术的主流方法，引领该领域朝着环境友好的方向发展。近期，在对叶绿体基因组进行工程改造以实现抗除草剂、抗虫、抗病、抗旱以及生产生物制药方面取得的成功，开启了生物技术的新纪元。成功对番茄有色体进行工程改造，使其在果实中实现高水平转基因表达，并与疫苗抗原的超表达相结合，以及使用植物源无抗生素选择标记，这对于口服递送目前最急需人群难以获得的可食用疫苗和生物制药而言，是个好兆头。
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本文引用的文献
1
Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts.一种可生物降解的蛋白质基聚合物在烟草叶绿体中的稳定表达。
Plant Cell Rep. 2000 Jan;19(3):257-262. doi: 10.1007/s002990050008.
2
Transient expression of β-glucuronidase in different cellular compartments following biolistic delivery of foreign DNA into wheat leaves and calli.生物弹射击入小麦叶片和愈伤组织中外源 DNA 后β-葡糖苷酸酶在不同细胞区室中的瞬时表达。
Plant Cell Rep. 1991 Mar;9(11):615-9. doi: 10.1007/BF00231800.
3
Computer-aided process analysis and economic evaluation for biosynthetic human insulin production-A case study.生物合成人胰岛素生产的计算机辅助过程分析与经济评估——案例研究
Biotechnol Bioeng. 1995 Dec 5;48(5):529-41. doi: 10.1002/bit.260480516.
4
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.烟草叶绿体基因组的完整核苷酸序列：其基因组织与表达
EMBO J. 1986 Sep;5(9):2043-2049. doi: 10.1002/j.1460-2075.1986.tb04464.x.
5
Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi.通过叶绿体基因组表达抗菌肽以控制植物致病细菌和真菌。
Plant Physiol. 2001 Nov;127(3):852-62.
6
Targeting a nuclear anthranilate synthase alpha-subunit gene to the tobacco plastid genome results in enhanced tryptophan biosynthesis. Return of a gene to its pre-endosymbiotic origin.将一个核邻氨基苯甲酸合酶α亚基基因靶向烟草质体基因组可增强色氨酸的生物合成。一个基因回归其共生前的起源。
Plant Physiol. 2001 Sep;127(1):131-41. doi: 10.1104/pp.127.1.131.
7
Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit.番茄质体的稳定遗传转化及外源蛋白在果实中的表达
Nat Biotechnol. 2001 Sep;19(9):870-5. doi: 10.1038/nbt0901-870.
8
Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts.天然霍乱毒素B亚基基因在转基因烟草叶绿体中的表达及组装成功能性寡聚体。
J Mol Biol. 2001 Aug 31;311(5):1001-9. doi: 10.1006/jmbi.2001.4921.
9
Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.利用CRE-lox位点特异性重组系统从质体基因组中高效去除选择标记基因。
Plant J. 2001 Jul;27(2):171-8. doi: 10.1046/j.1365-313x.2001.01068.x.
10
Multiple pathways for Cre/lox-mediated recombination in plastids.质体中Cre/lox介导的重组的多种途径。
Plant J. 2001 Jul;27(2):161-70. doi: 10.1046/j.1365-313x.2001.01067.x.
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