Guda C, Lee S-B, Daniell H
Molecular Genetics Program, Department of Botany and Microbiology, 101, Life Sciences Building, Auburn University, Auburn, AL 36849-5407 and Department of Molecular Biology and Microbiology, BIO 306, University of Central Florida, Orlando FL 32816-2360, USA e-mail:
Plant Cell Rep. 2000 Jan;19(3):257-262. doi: 10.1007/s002990050008.
Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.
基于生物弹性蛋白的聚合物（PBP）有多种医学应用（预防术后粘连）和非医学应用（可生物降解塑料）。本研究比较了整合到核基因组或叶绿体基因组的大单拷贝（LSC）或反向重复（IR）区域中的PBP基因（合成基因）在转基因烟草植株中的表达水平。聚合物转录本在IR植株中的积累量比核转基因植株高100倍。仅在IR转基因植株中观察到外源基因整合到所有叶绿体基因组中（同质体）以及聚合物转录本的更高水平，而在LSC转基因植株中未观察到。通过蛋白质免疫印迹分析进一步证实了聚合物蛋白的表达。
