Laboratório de Genética e Citogenética Animal, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE Brazil.
Genet Mol Biol. 2009 Oct;32(4):748-52. doi: 10.1590/S1415-47572009005000095. Epub 2009 Dec 1.
Phyllostomidae comprises the most diverse family of neotropical bats, its wide range of morphological features leading to uncertainty regarding phylogenetic relationships. Seeing that cytogenetics is one of the fields capable of providing support for currently adopted classifications through the use of several markers, a comparative analysis between two Phyllostomidae species was undertaken in the present study, with a view to supplying datasets for the further establishment of Phyllostomidae evolutionary relationships. Karyotypes of Lonchorhina aurita (2n = 32; FN = 60) and Trachops cirrhosus (2n = 30; FN = 56) were analyzed by G- and C-banding, silver nitrate staining (Ag-NOR) and base-specific fluorochromes. Chromosomal data obtained for both species are in agreement with those previously described, except for X chromosome morphology in T. cirrhosus, hence indicating chromosomal geographical variation in this species. A comparison of G-banding permitted the identification of homeologies in nearly all the chromosomes. Furthermore, C-banding and Ag-NOR patterns were comparable to what has already been observed in the family. In both species CMA(3) /DA/DAPI staining revealed an R-banding-like pattern with CMA (3) , whereas DAPI showed uniform staining in all the chromosomes. Fluorochrome staining patterns for pericentromeric constitutive heterochromatin (CH) regions, as well as for nucleolar organizing regions (NORs), indicated heterogeneity regarding these sequences among Phyllostomidae species.
叶口蝠科是新热带区最具多样性的蝙蝠科，其形态特征广泛，导致系统发育关系不确定。鉴于细胞遗传学是能够通过使用多个标记为当前采用的分类提供支持的领域之一，本研究对两种叶口蝠科物种进行了比较分析，以期为进一步建立叶口蝠科进化关系提供数据集。通过 G 带和 C 带、银染（Ag-NOR）和碱基特异性荧光染料对 Lonchorhina aurita（2n = 32；FN = 60）和 Trachops cirrhosus（2n = 30；FN = 56）的核型进行了分析。两种物种的染色体数据与先前描述的一致，但 T. cirrhosus 的 X 染色体形态除外，这表明该物种存在染色体地理变异。G 带比较允许鉴定几乎所有染色体的同源性。此外，C 带和 Ag-NOR 模式与该科已经观察到的模式相似。在两种物种中，CMA(3)/DA/DAPI 染色显示出与 CMA(3) 相似的 R 带模式，而 DAPI 在所有染色体上均显示均匀染色。着丝粒周围组成型异染色质（CH）区和核仁组织区（NOR）的荧光染料染色模式表明，这些序列在叶口蝠科物种之间存在异质性。
