Mecsas Joan
Department of Molecular Biology and Microbiology, 136 Harrison Avenue, Tufts University School of Medicine, Boston, MA 02111, USA.
Curr Opin Microbiol. 2002 Feb;5(1):33-7. doi: 10.1016/s1369-5274(02)00282-5.
From 2000-2001, over 10 studies have been completed, using signature-tagged mutagenesis, on a variety of bacterial pathogens. Investigators are examining the attenuated strains in additional in vivo, cell culture or in vitro assays to further characterize and catalog the mutants. Notable advances included screening of the attenuated mutants en masse in other assays, verification that mutant strains are defective for growth in specific host tissues, and identification of potential live-attenuated vaccine candidates. In addition, researchers are using signature-tagged strains to characterize the infection process, which is providing snapshots of bottlenecks and quantitative measurements of bacterial spread from tissue to tissue. This review focuses on themes emerging from signature-tagged mutagenesis studies completed on bacterial pathogens since mid-2000.
从2000年到2001年，已经完成了10多项使用签名标记诱变技术对多种细菌病原体进行的研究。研究人员正在额外的体内、细胞培养或体外试验中检测减毒株，以进一步对突变体进行表征和编目。显著进展包括在其他试验中对减毒突变体进行大规模筛选，验证突变菌株在特定宿主组织中生长存在缺陷，以及鉴定潜在的减毒活疫苗候选物。此外，研究人员正在使用签名标记菌株来表征感染过程，这为瓶颈环节提供了快照，并对细菌在组织间的传播进行了定量测量。本综述重点关注自2000年年中以来对细菌病原体完成的签名标记诱变研究中出现的主题。
