Słotwińska-Palugniok E, Wilczok T
Neoplasma. 1979;26(4):461-9.
DNA was isolated from livers of the rats treated with DAB during various steps of hepatoma development. After histological examination the tumor tissue was separated from the normal liver tissue and used as the source of DNA. Chromatographic fractionation on DEAE and Ecteola celluloses shows characteristic patterns for DNA isolated at various steps of hepatoma development. The largest differences in hepatoma DNA as compared to normal liver DNA were demonstrated in the DNA fraction eluated with 2.0 M-NaCl and NH3, gradient 0.1--1.0 M (m. w. 2--9 x 10(6)), and an increase in the first DNA fraction (m. w. less than 1 x 10(6)) was observed. Differences in the chromatographic patterns are discussed in terms of direct DAB action on DNA.
在肝癌发展的不同阶段，从经二乙基亚硝胺（DAB）处理的大鼠肝脏中分离出DNA。经过组织学检查后，将肿瘤组织与正常肝脏组织分离，并用作DNA的来源。在二乙氨基乙基（DEAE）纤维素和乙基纤维素上进行色谱分级分离，显示出在肝癌发展的不同阶段分离出的DNA的特征模式。与正常肝脏DNA相比，肝癌DNA最大的差异出现在用2.0M氯化钠和0.1 - 1.0M氨梯度洗脱的DNA组分中（分子量为2 - 9×10⁶），并且观察到第一个DNA组分（分子量小于1×10⁶）有所增加。根据二乙基亚硝胺对DNA的直接作用来讨论色谱模式的差异。
