通过DNase I抑制试验测定凝血酶刺激的血小板中肌动蛋白的重组。
Reorganization of actin in platelets stimulated by thrombin as measured by the DNase I inhibition assay.
作者信息
Carlsson L, Markey F, Blikstad I, Persson T, Lindberg U
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6376-80. doi: 10.1073/pnas.76.12.6376.
Abstract
The effect of thrombin stimulation on actin organization in human platelets has been analyzed by using the DNase I inhibition assay, which is selective for unpolymerized and filamentous actin. The results provide biochemical evidence for the suggestion that stimulation leads to rapid polymerization of actin. The measurements also reveal changes in the polymerization state of actin occurring after cell lysis. These changes are influenced by the concentration of free calcium in the extracts.
摘要
通过使用对未聚合和丝状肌动蛋白具有选择性的DNase I抑制试验，分析了凝血酶刺激对人血小板中肌动蛋白组织的影响。结果为刺激导致肌动蛋白快速聚合的观点提供了生化证据。这些测量还揭示了细胞裂解后肌动蛋白聚合状态的变化。这些变化受提取物中游离钙浓度的影响。
相似文献
1
Reorganization of actin in platelets stimulated by thrombin as measured by the DNase I inhibition assay.通过DNase I抑制试验测定凝血酶刺激的血小板中肌动蛋白的重组。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6376-80. doi: 10.1073/pnas.76.12.6376.
2
Tyrosine kinases activate store-mediated Ca2+ entry in human platelets through the reorganization of the actin cytoskeleton.酪氨酸激酶通过肌动蛋白细胞骨架的重组激活人血小板中储存介导的钙离子内流。
Biochem J. 2000 Oct 15;351 Pt 2(Pt 2):429-37.
3
Fibronectin potentiates actin polymerization in thrombin-activated platelets.纤连蛋白可增强凝血酶激活的血小板中的肌动蛋白聚合作用。
J Cell Biochem. 1986;30(1):71-7. doi: 10.1002/jcb.240300108.
4
Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.
Cell. 1978 Nov;15(3):935-43. doi: 10.1016/0092-8674(78)90277-5.
5
Concanavalin A induces interactions between surface glycoproteins and the platelet cytoskeleton.伴刀豆球蛋白A诱导表面糖蛋白与血小板细胞骨架之间的相互作用。
J Cell Biol. 1982 Feb;92(2):565-73. doi: 10.1083/jcb.92.2.565.
6
Dynamics of membrane-cytoskeleton interactions in activated blood platelets.活化血小板中膜-细胞骨架相互作用的动力学
Biochemistry. 1982 Jun 8;21(12):2825-32. doi: 10.1021/bi00541a003.
7
Cytoskeletal changes in platelets induced by thrombin and phorbol myristate acetate (PMA).凝血酶和佛波酯（PMA）诱导的血小板细胞骨架变化
Cell Biol Int. 1998;22(6):429-35. doi: 10.1006/cbir.1998.0271.
8
Characterization of platelet extracts before and after stimulation with respect to the possible role of profilactin as microfilament precursor.关于肌动蛋白原作为微丝前体的可能作用，对刺激前后的血小板提取物进行表征。
Cell. 1981 Jan;23(1):145-53. doi: 10.1016/0092-8674(81)90279-8.
9
Annexin V relocates to the platelet cytoskeleton upon activation and binds to a specific isoform of actin.膜联蛋白V在激活后重新定位于血小板细胞骨架，并与肌动蛋白的一种特定异构体结合。
Eur J Biochem. 2000 Aug;267(15):4720-30. doi: 10.1046/j.1432-1327.2000.01525.x.
10
The state of actin in activated human platelets.
Eur J Biochem. 1981 May 15;116(2):293-6. doi: 10.1111/j.1432-1033.1981.tb05332.x.
引用本文的文献
1
State of the art in platelet function testing.血小板功能检测的最新技术进展。
Transfus Med Hemother. 2013 Apr;40(2):73-86. doi: 10.1159/000350469. Epub 2013 Mar 18.
2
Regulation of LIM-kinase 1 and cofilin in thrombin-stimulated platelets.凝血酶刺激的血小板中LIM激酶1和丝切蛋白的调控
Blood. 2006 Jan 15;107(2):575-83. doi: 10.1182/blood-2004-11-4377. Epub 2005 Oct 11.
3
Signal-mediated depolymerization of actin in pollen during the self-incompatibility response.自交不亲和反应过程中花粉肌动蛋白的信号介导解聚
Plant Cell. 2002 Oct;14(10):2613-26. doi: 10.1105/tpc.002998.
4
Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes.血小板主要在分化的巨核细胞产生的前血小板突起末端组装而成。
J Cell Biol. 1999 Dec 13;147(6):1299-312. doi: 10.1083/jcb.147.6.1299.
5
Microtubule coils versus the surface membrane cytoskeleton in maintenance and restoration of platelet discoid shape.微管盘绕与血小板盘状形态维持及恢复中的表面膜细胞骨架
Am J Pathol. 1998 Feb;152(2):597-609.
6
Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein.凝溶胶蛋白和封端蛋白对血小板肌动蛋白丝倒刺端的协同调节。
J Cell Biol. 1996 Jul;134(2):389-99. doi: 10.1083/jcb.134.2.389.
7
Modulation of the interaction between G-actin and thymosin beta 4 by the ATP/ADP ratio: possible implication in the regulation of actin dynamics.ATP/ADP 比值对 G-肌动蛋白与胸腺素β4 之间相互作用的调节：对肌动蛋白动力学调节的潜在影响
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5034-8. doi: 10.1073/pnas.90.11.5034.
8
Snake venom cardiotoxin can rapidly induce actin polymerization in intact platelets.蛇毒心脏毒素可迅速诱导完整血小板中的肌动蛋白聚合。
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):591-4. doi: 10.1042/bj2900591.
9
Isolation and characterization of actin and actin-binding protein from human platelets.人血小板中肌动蛋白及肌动蛋白结合蛋白的分离与特性研究
J Cell Biol. 1981 Oct;91(1):201-11. doi: 10.1083/jcb.91.1.201.
10
Cytochalasins inhibit arachidonic acid metabolism in thrombin-stimulated platelets.细胞松弛素抑制凝血酶刺激的血小板中的花生四烯酸代谢。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7709-13. doi: 10.1073/pnas.79.24.7709.
本文引用的文献
1
Molecular weight and amino acid composition of deoxyribonuclease I.脱氧核糖核酸酶I的分子量及氨基酸组成
Biochemistry. 1967 Jan;6(1):335-42. doi: 10.1021/bi00853a050.
2
Fine structural alterations induced in platelets by adenosine diphosphate.二磷酸腺苷诱导血小板产生的超微结构改变
Blood. 1968 May;31(5):604-22.
3
Secretory mechanisms. Behaviour of adenine nucleotides during the platelet release reaction induced by adenosine diphosphate and adrenaline.分泌机制。二磷酸腺苷和肾上腺素诱导血小板释放反应过程中腺嘌呤核苷酸的行为。
Biochem J. 1972 Aug;129(1):67-82. doi: 10.1042/bj1290067.
4
The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm.肌动蛋白的聚合作用：其在某些棘皮动物精子顶体突起形成过程中的作用。
J Cell Biol. 1973 Oct;59(1):109-26. doi: 10.1083/jcb.59.1.109.
5
Cell communication, calcium ion, and cyclic adenosine monophosphate.细胞通讯、钙离子和环磷酸腺苷
Science. 1970 Oct 23;170(3956):404-12. doi: 10.1126/science.170.3956.404.
6
Studies on thrombosthenin a, the actin-like moiety of the contractile protein from blood platelets. I. Isolation, characterization and evidence for two forms of thrombosthenin A.关于血小板收缩蛋白中肌动蛋白样部分血栓收缩蛋白A的研究。I. 血栓收缩蛋白A两种形式的分离、特性鉴定及证据。
Biochim Biophys Acta. 1972 Oct 31;278(3):577-84. doi: 10.1016/0005-2795(72)90017-7.
7
The blood platelet release reaction.血小板释放反应。
Scand J Haematol Suppl. 1969;8:3-26.
8
Factors influencing platelet function: adhesion, release, and aggregation.影响血小板功能的因素：黏附、释放和聚集。
Pharmacol Rev. 1970 Jun;22(2):97-187.
9
Nucleotide leakage from platelets in artificial media: prevention by albumin and other macromolecules and relation to ADP-induced platelet aggregation.
Scand J Haematol. 1973;11(3):241-8. doi: 10.1111/j.1600-0609.1973.tb00124.x.
10
Electron microscopical observations on actinoid and myosinoid filaments in blood platelets.血小板中肌动蛋白样丝和肌球蛋白样丝的电子显微镜观察
J Ultrastruct Res. 1971 Nov;37(3):351-69. doi: 10.1016/s0022-5320(71)80129-6.
Zotero 插件