Analysis of long-chain acids or human plasma phosphatidylcholines (lecithins) and cholesteryl esters by glass open tubular capillary column gas chromatography for stroke patients and for normal subjects.

Methods are described for long-chain acid compositional analyses of human plasma phosphatidylcholines and plasma cholesteryl esters. Conventional procedures were employed for the isolation of these lipids and for the preparation of methyl esters of the acids. The gas chromatographic analytical procedure was based upon the use of thermostable glass open tubular capillary columns with a new polar phase. The methods were used in a study of plasma lipids in stroke patients and in normal young adult subjects.