Ingalls S T, Kriaris M S, Xu Y, DeWulf D W, Tserng K Y, Hoppel C L
Medical Research 151 (W), Department of Veterans Affairs Medical Center, Cleveland, OH 44106.
J Chromatogr. 1993 Sep 8;619(1):9-19. doi: 10.1016/0378-4347(93)80441-6.
A method is described for isolation from human plasma of non-esterified fatty acids, cholesteryl esters, triglycerides, cholesterol and diglycerides, monoglycerides, and some phospholipids by extraction and silica gel column chromatography. All of these lipid classes except diglycerides and cholesterol were separated cleanly in seven elution steps. Diglycerides and cholesterol were isolated together. Recovery of model compounds which represent the most significant classes of plasma lipids during the column chromatographic step was nearly complete. The overall recovery of added heptadecanoic acid from plasma specimens was 81% after both sample isolation steps. The overall recovery of added synthetic pentadecanoic acid and heptadecanoic acid ester lipid homologues from plasma was 80-91% after both sample preparation steps. About 6 h are required for extraction and isolation in duplicate of these lipid classes from twenty plasma specimens. Alternatively, non-esterified fatty acids can be isolated from twenty plasma specimens in duplicate within 4 h by a variation of the full procedure.
本文描述了一种通过萃取和硅胶柱色谱法从人血浆中分离非酯化脂肪酸、胆固醇酯、甘油三酯、胆固醇、甘油二酯、甘油单酯和一些磷脂的方法。除甘油二酯和胆固醇外，所有这些脂质类别均在七个洗脱步骤中被干净地分离。甘油二酯和胆固醇一起被分离出来。在柱色谱步骤中，代表血浆脂质最重要类别的模型化合物的回收率几乎是完全的。经过两个样品分离步骤后，血浆标本中添加的十七烷酸的总回收率为81%。经过两个样品制备步骤后，血浆中添加的合成十五烷酸和十七烷酸酯脂质同系物的总回收率为80-91%。从二十个血浆标本中重复提取和分离这些脂质类别大约需要6小时。或者，通过对完整程序的变通，可在4小时内从二十个血浆标本中重复分离非酯化脂肪酸。
