da Silva Arnaldo, Kawazoe Urara, Freitas Fabio F T, Gatti Maria Silvia V, Dolder Heidi, Schumacher Robert Ivan, Juliano Maria Aparecida, da Silva Marcio Jose, Leite Adilson
Centro de Biologia Molecular e Engenharia Genetica, Universidade Estadual de Campinas, Cidade Universitaria Zeferino Vaz, 13083-970, SP, Campinas, Brazil.
Mol Biochem Parasitol. 2002 Mar;120(1):53-60. doi: 10.1016/s0166-6851(01)00439-x.
In the present work, we describe the discovery of PW2, a novel peptide presenting in vitro activity against Eimeria acervulina and E. tenella sporozoites. PW2 was selected from phage display (Ph.D.) peptide libraries by an alternative method of panning using living purified E. acervulina sporozoites as targets. Our results showed that the peptide disrupts the sporozoite pellicle, resembling the effect caused by most natural antimicrobial peptides. PW2 peptide was also effective against fungi and showed low activity against Toxoplasma gondii tachyzoites, but no activity against Trypanosoma cruzi, Crithidia fasciculata epimastigotes, and bacteria. Additionally, the parasiticidal concentrations of PW2 produced a very low lytic effect on mammalian and avian cells. The effectiveness against Eimeria sporozoites and the absence of adverse effects to host cells indicates that PW2 may be used as a model to generate new drugs for the control of avian coccidiosis.
在本研究中，我们描述了PW2的发现，PW2是一种对堆型艾美耳球虫和柔嫩艾美耳球虫子孢子具有体外活性的新型肽。PW2是通过一种替代淘选方法，以活的纯化堆型艾美耳球虫子孢子为靶标，从噬菌体展示（Ph.D.）肽库中筛选出来的。我们的结果表明，该肽会破坏子孢子的表膜，类似于大多数天然抗菌肽所产生的效果。PW2肽对真菌也有效，对刚地弓形虫速殖子活性较低，但对克氏锥虫、束状短膜虫前鞭毛体和细菌无活性。此外，PW2的杀寄生虫浓度对哺乳动物和禽类细胞产生的溶细胞作用非常低。对艾美耳球虫子孢子的有效性以及对宿主细胞无不良影响表明，PW2可作为开发控制禽球虫病新药的模型。
