用于嗜热链球菌指数生长的最低化学成分限定培养基的开发。
Development of a minimal chemically-defined medium for the exponential growth of Streptococcus thermophilus.
作者信息
Letort C, Juillard V
机构信息
Unité de Recherches Laitières et Génétique Appliquée, INRA, Jouy-en-Josas, France.
出版信息
J Appl Microbiol. 2001 Dec;91(6):1023-9. doi: 10.1046/j.1365-2672.2001.01469.x.
AIMS
The present work aimed to define a minimal chemically-defined medium which could sustain the growth of most (if not all) strains of Streptococcus thermophilus.
METHODS AND RESULTS
A minimal medium containing 20 components, including one carbohydrate source, six amino acids, two metallic ions, six vitamins and urea allowed for growth of 13 out of 15 Strep. thermophilus strains. Growth of the two last strains required the presence of additional amino acids, the number of which depended on the strain. Growth rates of the strains in the minimal medium ranged from 0.38 to 0.64 h(-1), and final populations were about 10(8) cfu ml(-1).
CONCLUSIONS
Streptococcus thermophilus appears much less demanding than other lactic acid bacteria.
SIGNIFICANCE AND IMPACT OF THE STUDY
The definition of such a growth medium will be very useful for metabolic flux studies as well as peptide transport studies.
目的
本研究旨在确定一种能维持大多数（即便不是全部）嗜热链球菌菌株生长的最低化学成分限定培养基。
方法与结果
一种包含20种成分的最低培养基，其中包括一种碳水化合物源、六种氨基酸、两种金属离子、六种维生素和尿素，可使15株嗜热链球菌中的13株生长。最后两株菌株的生长需要额外的氨基酸，所需氨基酸数量因菌株而异。这些菌株在最低培养基中的生长速率范围为0.38至0.64 h⁻¹，最终菌数约为10⁸ cfu/ml。
结论
嗜热链球菌对营养的需求似乎远低于其他乳酸菌。
研究的意义与影响
这种生长培养基的确定对于代谢通量研究以及肽转运研究将非常有用。
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