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组氨酸、异亮氨酸和谷氨酸对937游离胞外多糖生物合成的转录调控作用

The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of 937.

作者信息

Wa Yunchao, Zhao Xia, Zhang Chenchen, Qu Hengxian, Chen Dawei, Chen Xia, Huang Yujun, Gu Ruixia

机构信息

Key Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, China.

College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, China.

出版信息

Front Microbiol. 2025 Jan 8;15:1476940. doi: 10.3389/fmicb.2024.1476940. eCollection 2024.

DOI:10.3389/fmicb.2024.1476940
PMID:39845036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11751036/
Abstract

INTRODUCTION

The free exopolysaccharide (f-EPS) produced by is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.

METHODS

In this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of 937.

RESULTS

The mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of . The upregulation of , , and transcript levels was caused by the increase in histidine concentration. The upregulation of transcript levels of genes related to phenylalanine/tyrosine/tryptophan/histidine/ valine/leucine/isoleucine synthesis was caused by the increase in isoleucine concentrations.

DISCUSSION

This indicates that 3 amino acids have different mechanisms for enhancing the biosynthesis of f-EPS in 937.

摘要

引言

[具体微生物名称]产生的游离胞外多糖（f-EPS）是一种天然的质地改良剂，具有多种益生元活性。我们之前的研究表明，在化学限定培养基中存在15 mM的谷氨酸、异亮氨酸和组氨酸时，[具体微生物名称] 937的f-EPS产量增加了2倍。

方法

在本研究中，我们使用转录组学和定量聚合酶链反应（qPCR）进一步探究3种氨基酸对[具体微生物名称] 937的f-EPS生物合成增强作用的具体机制。

结果

mRNA测序分析和靶向途径分析表明，在化学限定培养基（CDM）中，随着组氨酸、异亮氨酸和谷氨酸浓度的增加，[具体微生物名称] 937中与组氨酸/缬氨酸/亮氨酸/异亮氨酸/苯丙氨酸/酪氨酸/色氨酸合成、半乳糖代谢、嘌呤代谢和群体感应相关的基因显著上调。qPCR结果表明，半乳糖代谢和核苷酸糖合成相关基因的显著上调归因于谷氨酸浓度的增加，并且谷氨酸可以诱导[相关基因名称]的表达。[相关基因名称]、[相关基因名称]和[相关基因名称]转录水平的上调是由组氨酸浓度的增加引起的。与苯丙氨酸/酪氨酸/色氨酸/组氨酸/缬氨酸/亮氨酸/异亮氨酸合成相关的基因转录水平的上调是由异亮氨酸浓度的增加引起的。

讨论

这表明3种氨基酸在增强[具体微生物名称] 937中f-EPS生物合成方面具有不同的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/1ff141a0e994/fmicb-15-1476940-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/3e5471cacafb/fmicb-15-1476940-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/8716a47f4f5d/fmicb-15-1476940-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/d2acffd1ec27/fmicb-15-1476940-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/431daa17528d/fmicb-15-1476940-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/ab62fe0aacad/fmicb-15-1476940-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/9220293ee782/fmicb-15-1476940-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/6476b51d6275/fmicb-15-1476940-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/c1556ed2485c/fmicb-15-1476940-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/1ff141a0e994/fmicb-15-1476940-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/3e5471cacafb/fmicb-15-1476940-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/8716a47f4f5d/fmicb-15-1476940-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/d2acffd1ec27/fmicb-15-1476940-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/431daa17528d/fmicb-15-1476940-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/ab62fe0aacad/fmicb-15-1476940-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/9220293ee782/fmicb-15-1476940-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/6476b51d6275/fmicb-15-1476940-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/c1556ed2485c/fmicb-15-1476940-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ff8/11751036/1ff141a0e994/fmicb-15-1476940-g009.jpg

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Effect of amino acids on free exopolysaccharide biosynthesis by Streptococcus thermophilus 937 in chemically defined medium.氨基酸对嗜热链球菌 937 在化学成分确定的培养基中合成游离胞外多糖的影响。
J Dairy Sci. 2022 Aug;105(8):6460-6468. doi: 10.3168/jds.2022-21814. Epub 2022 Jun 9.
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