Yamaoka Y, Kita M, Kodama T, Sawai N, Kashima K, Imanishi J
Third Dept. of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Scand J Gastroenterol. 1995 Dec;30(12):1153-9. doi: 10.3109/00365529509101624.
We have studied the cytokine production patterns in gastric mucosal biopsy specimens with and without the Helicobacter pylori infection, using a reverse transcription-polymerase chain reaction (RT-PCR) method capable of detecting low levels of specific mRNA.
Total RNA was prepared from biopsy specimens with the acid guanidinium thiocyanate-phenol-chloroform method. cDNA was synthesized by M-MLV RTase and amplified using the oligonucleotide primers specific for interleukin-1 beta (IL-1beta), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, tumor necrosis factor-alpha (TNF-alpha), interferon-alpha (IFN-alpha), IFN-beta, and IFN-gamma by PCR methods.
Although IL-1 beta and IFN-gamma mRNA were detected in most specimens, IL-2, IL-3, IL-4, IL-5, IL-9, TNF-alpha, and IFN-beta mRNA were not detected at all. The expressions of IL-7 and IL-8 mRNA were significantly higher in H. pylori-positive gastritis than in H. pylori-negative normal controls. There was a significant correlation between the expression of IL-8 mRNA and the severity of gastritis both in the antrum and in the corpus. On the other hand, there was a significant correlation between the expression of IL-7 mRNA and the severity of gastritis only in the corpus.
These findings suggest that some cytokines, especially IL-7 and IL-8, play some roles in H. pylori-associated gastritis.
我们使用一种能够检测低水平特异性mRNA的逆转录-聚合酶链反应（RT-PCR）方法，研究了有无幽门螺杆菌感染的胃黏膜活检标本中的细胞因子产生模式。
采用酸性异硫氰酸胍-苯酚-氯仿法从活检标本中提取总RNA。通过M-MLV逆转录酶合成cDNA，并使用针对白细胞介素-1β（IL-1β）、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、肿瘤坏死因子-α（TNF-α）、干扰素-α（IFN-α）、IFN-β和IFN-γ的寡核苷酸引物，通过PCR方法进行扩增。
虽然在大多数标本中检测到了IL-1β和IFN-γ mRNA，但未检测到IL-2、IL-3、IL-4、IL-5、IL-9、TNF-α和IFN-β mRNA。幽门螺杆菌阳性胃炎中IL-7和IL-8 mRNA的表达明显高于幽门螺杆菌阴性正常对照。IL-8 mRNA的表达与胃窦和胃体部胃炎的严重程度之间存在显著相关性。另一方面，IL-7 mRNA的表达与胃炎严重程度之间仅在胃体部存在显著相关性。
这些发现表明某些细胞因子，尤其是IL-7和IL-8，在幽门螺杆菌相关性胃炎中发挥了一定作用。
