白泻根根中葫芦烷糖苷的抗炎和抗肿瘤促进作用

Anti-inflammatory and anti-tumor-promoting effects of cucurbitane glycosides from the roots of Bryonia dioica.

作者信息

Ukiya Motohiko, Akihisa Toshihiro, Yasukawa Ken, Tokuda Harukuni, Toriumi Masakazu, Koike Kazuo, Kimura Yumiko, Nikaido Tamotsu, Aoi Wataru, Nishino Hoyoku, Takido Michio

机构信息

College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.

出版信息

J Nat Prod. 2002 Feb;65(2):179-83. doi: 10.1021/np010423u.

DOI:10.1021/np010423u

PMID:11858752

Abstract

Seven new triterpene glycosides, bryoniosides A-G (1-7), have been isolated along with two known triterpene glycosides, cabenoside D (8) and bryoamaride (9), from a methanol extract of the roots of Bryoniadioica. The structures of 1-7 were determined on the basis of spectroscopic and chemical methods. Six compounds, 2, 3, 5, and 7-9, and 11 compounds, 1-9, bryodulcosigenin (10), and bryosigenin (11), respectively, were evaluated for their inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1 microg/ear) in mice and on Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA. All compounds tested showed marked anti-inflammatory effects, with 50% inhibitory doses (ID(50)) of 0.2-0.6 mg per ear. In addition, all of the compounds tested except for compound 5 showed potent inhibitory effects on EBV-EA induction (100% inhibition at 1 x 10(3) mol ratio/TPA).

摘要

从白泻根的根甲醇提取物中分离出七种新的三萜糖苷，即白泻根苷A - G（1 - 7），以及两种已知的三萜糖苷，卡贝诺苷D（8）和白泻根酰胺（9）。通过光谱和化学方法确定了1 - 7的结构。分别评估了六种化合物，即2、3、5、7 - 9，以及十一种化合物，即1 - 9、白泻根甜苷元（10）和白泻根苷元（11）对小鼠12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的炎症（1微克/耳）以及对TPA诱导的爱泼斯坦 - 巴尔病毒早期抗原（EBV - EA）激活的抑制作用。所有测试化合物均显示出显著的抗炎作用，每耳的50%抑制剂量（ID50）为0.2 - 0.6毫克。此外，除化合物5外，所有测试化合物均对EBV - EA诱导显示出强效抑制作用（在1×10³摩尔比/TPA时100%抑制）。