Ukiya Motohiko, Akihisa Toshihiro, Yasukawa Ken, Tokuda Harukuni, Toriumi Masakazu, Koike Kazuo, Kimura Yumiko, Nikaido Tamotsu, Aoi Wataru, Nishino Hoyoku, Takido Michio
College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.
J Nat Prod. 2002 Feb;65(2):179-83. doi: 10.1021/np010423u.
Seven new triterpene glycosides, bryoniosides A-G (1-7), have been isolated along with two known triterpene glycosides, cabenoside D (8) and bryoamaride (9), from a methanol extract of the roots of Bryoniadioica. The structures of 1-7 were determined on the basis of spectroscopic and chemical methods. Six compounds, 2, 3, 5, and 7-9, and 11 compounds, 1-9, bryodulcosigenin (10), and bryosigenin (11), respectively, were evaluated for their inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1 microg/ear) in mice and on Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA. All compounds tested showed marked anti-inflammatory effects, with 50% inhibitory doses (ID(50)) of 0.2-0.6 mg per ear. In addition, all of the compounds tested except for compound 5 showed potent inhibitory effects on EBV-EA induction (100% inhibition at 1 x 10(3) mol ratio/TPA).
从白泻根的根甲醇提取物中分离出七种新的三萜糖苷，即白泻根苷A - G（1 - 7），以及两种已知的三萜糖苷，卡贝诺苷D（8）和白泻根酰胺（9）。通过光谱和化学方法确定了1 - 7的结构。分别评估了六种化合物，即2、3、5、7 - 9，以及十一种化合物，即1 - 9、白泻根甜苷元（10）和白泻根苷元（11）对小鼠12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的炎症（1微克/耳）以及对TPA诱导的爱泼斯坦 - 巴尔病毒早期抗原（EBV - EA）激活的抑制作用。所有测试化合物均显示出显著的抗炎作用，每耳的50%抑制剂量（ID50）为0.2 - 0.6毫克。此外，除化合物5外，所有测试化合物均对EBV - EA诱导显示出强效抑制作用（在1×10³摩尔比/TPA时100%抑制）。
