Zargari A, Eshaghi H, Bäck O, Johansson S, Scheynius A
Ludwig Institute for Cancer Research, Unit of Yeast Molecular Genetic, Stockholm Branch, Sweden.
Acta Derm Venereol. 2001 Nov-Dec;81(6):418-22. doi: 10.1080/000155501317208363.
IgE reactivity to the opportunistic yeast Malassezia furfur can be found in patients with atopic dermatitis (AD). We have previously cloned and expressed 6 recombinant allergens (rMal f 1, rMal f 5-9) from M. furfur. In the present study, we used ImmunoCAP to investigate whether these rMal f allergens can be useful in the diagnosis of M. furfur-associated AD compared with the M. furfur extract. A total of 156 adult patients with a clinical diagnosis of AD participated in the study. Sixty-four percent had increased total serum IgE levels, 79% had specific IgE antibodies to common inhalant allergens and 47% had IgE antibodies to M. furfur extract. IgE antibodies to any of the rMal f allergens were detected among 86 (55%) of the patients, 14 (16%) of whom did not react to the M. furfur extract. Any individual rMal f allergen detected between 32% and 89% of the patients ImmunoCAP-positive to the M. furfur extract, with the highest sensitivity for rMal f 9. Therefore, a couple of individual rMal f allergens can improve the diagnosis of M. furfur-associated IgE allergies in patients with AD.
特应性皮炎（AD）患者体内可检测到对机会性酵母菌糠秕马拉色菌的IgE反应性。我们之前已从糠秕马拉色菌中克隆并表达了6种重组变应原（rMal f 1、rMal f 5 - 9）。在本研究中，我们使用免疫捕获法（ImmunoCAP）来研究与糠秕马拉色菌提取物相比，这些rMal f变应原是否有助于糠秕马拉色菌相关AD的诊断。共有156例临床诊断为AD的成年患者参与了该研究。64%的患者血清总IgE水平升高，79%的患者对常见吸入性变应原具有特异性IgE抗体，47%的患者对糠秕马拉色菌提取物具有IgE抗体。86例（55%）患者体内检测到针对任何一种rMal f变应原的IgE抗体，其中14例（16%）患者对糠秕马拉色菌提取物无反应。在对糠秕马拉色菌提取物免疫捕获法检测呈阳性的患者中，任何一种单独的rMal f变应原在32%至89%的患者中被检测到，其中rMal f 9的敏感性最高。因此，几种单独的rMal f变应原可改善AD患者中糠秕马拉色菌相关IgE过敏的诊断。
