School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.
Front Cell Infect Microbiol. 2021 Jan 21;10:561382. doi: 10.3389/fcimb.2020.561382. eCollection 2020.
species are a major part of the normal mycobiota and colonize mainly sebum-rich skin regions of the body. This group of fungi cause a variety of infections such as pityriasis versicolor, folliculitis, and fungaemia. In particular, and its allergens have been associated with non-infective inflammatory diseases such as seborrheic dermatitis and atopic eczema. The aim of this study was to investigate the host response to on oily skin (supplemented with oleic acid) and non-oily skin using an human skin model. Host-pathogen interactions were analyzed by SEM, histology, gene expression, immunoassays and dual species proteomics. The skin response to was characterized by increased expression of the genes encoding β-defensin 3 and RNase7, and by high levels of S100 proteins in tissue. Supplementation of oleic acid onto skin was associated with direct contact of yeasts with keratinocytes and epidermal damage. In oily conditions, there was increased expression of but no expression of antimicrobial peptide genes in the skin's response to . In supernatants from inoculated skin plus oleic acid, TNFα, IL-6, and IL1-β levels were decreased and IL-18 levels were significantly increased.
物种是正常真菌区系的主要组成部分，主要定植于富含皮脂的皮肤区域。这群真菌会引起各种感染，如花斑癣、毛囊炎和真菌血症。特别是，及其过敏原与非传染性炎症性疾病如脂溢性皮炎和特应性皮炎有关。本研究旨在使用人体皮肤模型研究宿主对油性皮肤（补充油酸）和非油性皮肤的反应。通过 SEM、组织学、基因表达、免疫测定和双种蛋白质组学分析宿主-病原体相互作用。皮肤对的反应表现为编码β-防御素 3 和 RNase7 的基因表达增加，以及组织中 S100 蛋白水平升高。在皮肤补充油酸后，酵母与角质形成细胞直接接触，并导致表皮损伤。在油性条件下，皮肤对的反应中表达增加，但抗菌肽基因无表达。在接种皮肤加油酸的上清液中，TNFα、IL-6 和 IL1-β 水平降低，IL-18 水平显著升高。
