• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪脑胚胎发育过程中的 Reelin 表达。

Reelin expression during embryonic development of the pig brain.

机构信息

Institute of Human Genetics, University of Aarhus, Bartholin Building 1240, Wilhelm Meyers Allé, 8000 Aarhus C, Denmark.

出版信息

BMC Neurosci. 2010 Jun 15;11:75. doi: 10.1186/1471-2202-11-75.

DOI:10.1186/1471-2202-11-75
PMID:20550682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2895594/
Abstract

BACKGROUND

Reelin is an extracellular glycoprotein of crucial importance in the developmental organisation of neurons in the mammalian cerebral cortex and other laminated brain regions. The pig possesses a gyrencephalic brain that bears resemblance to the human brain. In order to establish an animal model for neuronal migration disorders in the pig, we have studied the expression pattern and structure of Reelin during pig brain development.

RESULTS

We determined the sequence of pig Reelin mRNA and protein and identified a high degree of homology to human Reelin. A peak in Reelin mRNA and protein expression is present during the period of major neurogenesis and neuronal migration. This resembles observations for human brain development. Immunohistochemical analysis showed the highest expression of Reelin in the Cajal-Reztius cells of the marginal zone, in resemblance with observations for the developing brain in humans and other mammalian species.

CONCLUSIONS

We conclude that the pig might serve as an alternative animal model to study Reelin functions and that manipulation of the pig Reelin could allow the establishment of an animal model for human neuronal migration disorders.

摘要

背景

Reelin 是一种细胞外糖蛋白，在哺乳动物大脑皮层和其他层状脑区神经元的发育组织中具有至关重要的作用。猪具有脑回，与人类大脑相似。为了在猪中建立神经元迁移障碍的动物模型，我们研究了 Reelin 在猪脑发育过程中的表达模式和结构。

结果

我们确定了猪 Reelin mRNA 和蛋白质的序列，并鉴定出与人类 Reelin 高度同源。在主要神经发生和神经元迁移期间，Reelin mRNA 和蛋白质表达达到高峰。这类似于人类大脑发育的观察结果。免疫组织化学分析显示，Reelin 在边缘带的 Cajal-Reztius 细胞中的表达最高，与人类和其他哺乳动物发育中的大脑的观察结果相似。

结论

我们得出结论，猪可能是研究 Reelin 功能的替代动物模型，并且猪 Reelin 的操作可能允许建立人类神经元迁移障碍的动物模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/b27cb6f3cc23/1471-2202-11-75-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/3827665dad6f/1471-2202-11-75-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/87342db9c08f/1471-2202-11-75-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/b27cb6f3cc23/1471-2202-11-75-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/3827665dad6f/1471-2202-11-75-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/87342db9c08f/1471-2202-11-75-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d0b/2895594/b27cb6f3cc23/1471-2202-11-75-3.jpg

相似文献

1
Reelin expression during embryonic development of the pig brain.猪脑胚胎发育过程中的 Reelin 表达。
BMC Neurosci. 2010 Jun 15;11:75. doi: 10.1186/1471-2202-11-75.
2
Impaired Reelin-Dab1 Signaling Contributes to Neuronal Migration Deficits of Tuberous Sclerosis Complex.Reelin-Dab1信号通路受损导致结节性硬化症的神经元迁移缺陷。
Cell Rep. 2015 Aug 11;12(6):965-78. doi: 10.1016/j.celrep.2015.07.013. Epub 2015 Jul 30.
3
Proteolytic cleavage of transmembrane cell adhesion molecule L1 by extracellular matrix molecule Reelin is important for mouse brain development.细胞外基质分子 Reelin 通过蛋白水解切割跨膜细胞黏附分子 L1 对小鼠大脑发育很重要。
Sci Rep. 2017 Nov 10;7(1):15268. doi: 10.1038/s41598-017-15311-x.
4
Reelin Prevents Apical Neurite Retraction during Terminal Translocation and Dendrite Initiation.Reelin可防止顶端神经突在终末移位和树突起始过程中回缩。
J Neurosci. 2015 Jul 29;35(30):10659-74. doi: 10.1523/JNEUROSCI.1629-15.2015.
5
LIM-homeobox gene Lhx5 is required for normal development of Cajal-Retzius cells.LIM 同源盒基因 Lhx5 对于 Cajal-Retzius 细胞的正常发育是必需的。
J Neurosci. 2010 Aug 4;30(31):10551-62. doi: 10.1523/JNEUROSCI.5563-09.2010.
6
Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages.投射神经元中功能性Reelin受体的下调意味着Reelin的主要作用发生在早期/迁移前阶段。
J Neurosci. 2009 Aug 26;29(34):10653-62. doi: 10.1523/JNEUROSCI.0345-09.2009.
7
Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex.Reelin与Notch信号之间的相互作用调节大脑皮质中的神经元迁移。
Neuron. 2008 Oct 23;60(2):273-84. doi: 10.1016/j.neuron.2008.09.026.
8
Role for Reelin in stabilizing cortical architecture.Reelin 在稳定皮质结构中的作用。
Trends Neurosci. 2010 Sep;33(9):407-14. doi: 10.1016/j.tins.2010.06.001. Epub 2010 Jul 1.
9
[Functions of Reelin in cortical neuron migration].[Reelin在皮层神经元迁移中的作用]
Seikagaku. 2016 Feb;88(1):105-13.
10
Reelin controls neuronal positioning by promoting cell-matrix adhesion via inside-out activation of integrin α5β1.Reelin 通过激活整联蛋白 α5β1 的内-外激活作用促进细胞-基质黏附，从而控制神经元的定位。
Neuron. 2012 Oct 18;76(2):353-69. doi: 10.1016/j.neuron.2012.07.020. Epub 2012 Oct 17.

引用本文的文献

1
Developmental changes of Reelin-expressing cell populations in the marginal zone of the neocortex of the European wild boar, Sus scrofa.欧洲野猪（Sus scrofa）新皮质边缘区中表达Reelin的细胞群体的发育变化。
Brain Struct Funct. 2025 Jun 11;230(6):96. doi: 10.1007/s00429-025-02958-w.
2
Establishing the pig as a translational animal model for neurodevelopment.将猪确立为神经发育的转化动物模型。
Transl Neurosci. 2025 Apr 24;16(1):20250369. doi: 10.1515/tnsci-2025-0369. eCollection 2025 Jan 1.
3
The risks of RELN polymorphisms and its expression in the development of otosclerosis.

本文引用的文献

1
Molecular regulation of neuronal migration during neocortical development.新皮质发育过程中神经元迁移的分子调控
Mol Cell Neurosci. 2009 Sep;42(1):11-22. doi: 10.1016/j.mcn.2009.06.003. Epub 2009 Jun 10.
2
Developmental anatomy of reeler mutant mouse.reeler突变小鼠的发育解剖学
Dev Growth Differ. 2009 Apr;51(3):271-86. doi: 10.1111/j.1440-169X.2009.01102.x.
3
Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex.Reelin与Notch信号之间的相互作用调节大脑皮质中的神经元迁移。
RELN 多态性及其表达在耳硬化症发展中的风险。
PLoS One. 2022 Jun 3;17(6):e0269558. doi: 10.1371/journal.pone.0269558. eCollection 2022.
4
Cerebral organoids and their potential for studies of brain diseases in domestic animals.类脑器官及其在研究家畜脑部疾病中的潜力。
Vet Res. 2021 May 3;52(1):65. doi: 10.1186/s13567-021-00931-z.
5
Maternal viral infection causes global alterations in porcine fetal microglia.母体病毒感染导致猪胎儿小胶质细胞的全局改变。
Proc Natl Acad Sci U S A. 2019 Oct 1;116(40):20190-20200. doi: 10.1073/pnas.1817014116. Epub 2019 Sep 16.
6
Use of a Piglet Model for the Study of Anesthetic-induced Developmental Neurotoxicity (AIDN): A Translational Neuroscience Approach.使用仔猪模型研究麻醉诱导的发育性神经毒性（AIDN）：一种转化神经科学方法。
J Vis Exp. 2017 Jun 11(124):55193. doi: 10.3791/55193.
7
A novel, clinically relevant use of a piglet model to study the effects of anesthetics on the developing brain.一种用于研究麻醉剂对发育中大脑影响的新型仔猪模型的临床相关应用。
Clin Transl Med. 2016 Mar;5(1):2. doi: 10.1186/s40169-015-0079-9. Epub 2016 Jan 12.
8
Characterization of hippocampal Cajal-Retzius cells during development in a mouse model of Alzheimer's disease (Tg2576).阿尔茨海默病模型小鼠（Tg2576）中海马 Cajal-Retzius 细胞发育过程的特征。
Neural Regen Res. 2014 Feb 15;9(4):394-401. doi: 10.4103/1673-5374.128243.
9
The rs39335 polymorphism of the RELN gene is not associated with otosclerosis in a southern Italian population.RELN基因的rs39335多态性与意大利南部人群的耳硬化症无关。
Acta Otorhinolaryngol Ital. 2013 Oct;33(5):320-3.
10
Controversies in RELN/reelin expression in otosclerosis.耳硬化症中 RELN/reelin 表达的争议。
Eur Arch Otorhinolaryngol. 2012 Feb;269(2):431-40. doi: 10.1007/s00405-011-1653-4. Epub 2011 Jun 1.
Neuron. 2008 Oct 23;60(2):273-84. doi: 10.1016/j.neuron.2008.09.026.
4
Strange bedfellows: Reelin and Notch signaling interact to regulate cell migration in the developing neocortex.奇怪的组合：Reelin和Notch信号相互作用以调节发育中的新皮质中的细胞迁移。
Neuron. 2008 Oct 23;60(2):189-91. doi: 10.1016/j.neuron.2008.10.009.
5
Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse.小鼠大脑皮质组织发生过程中细胞迁移的放射自显影研究。
Nature. 1961 Nov 25;192:766-8. doi: 10.1038/192766b0.
6
The use of pigs in neuroscience: modeling brain disorders.猪在神经科学中的应用：模拟脑部疾病
Neurosci Biobehav Rev. 2007;31(5):728-51. doi: 10.1016/j.neubiorev.2007.02.003. Epub 2007 Mar 4.
7
Trekking across the brain: the journey of neuronal migration.穿越大脑：神经元迁移之旅
Cell. 2007 Jan 12;128(1):29-43. doi: 10.1016/j.cell.2006.12.021.
8
Recent progress in understanding the role of Reelin in radial neuronal migration, with specific emphasis on the dentate gyrus.在理解Reelin在放射状神经元迁移中的作用方面的最新进展，特别强调齿状回。
Eur J Neurosci. 2006 Feb;23(4):901-9. doi: 10.1111/j.1460-9568.2006.04612.x.
9
The reeler mouse: anatomy of a mutant.摇晃小鼠：突变体解剖学
Int Rev Neurobiol. 2005;71:383-417. doi: 10.1016/s0074-7742(05)71016-3.
10
Novel calretinin and reelin expressing neuronal population includes Cajal-Retzius-type cells in the neocortex of adult pigs.表达新型钙视网膜蛋白和Reelin的神经元群体包括成年猪新皮质中的Cajal-Retzius型细胞。
Neuroscience. 2005;136(1):217-30. doi: 10.1016/j.neuroscience.2005.07.039. Epub 2005 Sep 21.