利用计算机辅助精子分析评估镉对大鼠精子活力的影响。
Effects of cadmium on rat sperm motility evaluated with computer assisted sperm analysis.
作者信息
Xu L C, Wang S Y, Yang X F, Wang X R
机构信息
Key Laboratory for Applied Toxicology of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China.
出版信息
Biomed Environ Sci. 2001 Dec;14(4):312-7.
OBJECTIVE
To study effects of cadmium on rat sperm motility evaluated with computer assisted sperm analysis.
METHODS
Different doses of cadmium chloride (0.2, 0.4, 0.8 mg Cd/kg BW) were administrated i.p. to adult male Sprague-Dawley rats. Control animals received the same volume of 0.9% NaCl solution. After 7 days, the rats were sacrificed with their testes removed. A part of one testis was used for testicular sperm head counts and daily sperm production observation. The motility of spermatozoa obtained from cauda epididymides using the "diffusion" method was measured by computer assisted sperm analysis (CASA).
RESULTS
The sperm head counts and daily sperm production decreased significantly in the high dose group. The motility of spermatozoa in the middle dose group was reduced significantly. No motile sperm was found in the high dose group. The results suggest that germinal epithelium was impaired irreversibly in a short time to produce toxic effects on spermatogenesis at high cadmium doses.
CONCLUSION
Cadmium may reduce sperm motility at a dose far below the dose affecting sperm production at this time point. The motility of sperm is an early and sensitive endpoint for the assessment of cadmium toxicity on male reproduction.
目的
采用计算机辅助精子分析技术研究镉对大鼠精子活力的影响。
方法
将不同剂量的氯化镉（0.2、0.4、0.8毫克镉/千克体重）腹腔注射给成年雄性斯普拉格-道利大鼠。对照组动物注射相同体积的0.9%氯化钠溶液。7天后，处死大鼠并取出睾丸。取一侧睾丸的一部分用于睾丸精子头部计数和每日精子生成观察。采用“扩散”法从附睾尾部获取精子，通过计算机辅助精子分析（CASA）测量精子活力。
结果
高剂量组精子头部计数和每日精子生成显著减少。中剂量组精子活力显著降低。高剂量组未发现活动精子。结果表明，高镉剂量下生精上皮在短时间内受到不可逆损伤，对精子发生产生毒性作用。
结论
在本时间点，镉可能在远低于影响精子生成剂量的情况下降低精子活力。精子活力是评估镉对雄性生殖毒性的早期敏感指标。
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