Lim Seung Oe, Park Sung-Jun, Kim Won, Park Sung Gyoo, Kim Hie-Joon, Kim Yong-Il, Sohn Tae-Sung, Noh Jae-Hyung, Jung Guhung
School of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
Biochem Biophys Res Commun. 2002 Mar 8;291(4):1031-7. doi: 10.1006/bbrc.2002.6547.
Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC.
肝细胞癌（HCC）的发生是一个复杂的过程，涉及基因表达的多种变化，且通常发生在肝硬化的情况下。在本研究中，我们观察了从HCC患者肝脏分离出的三种组织类型（正常组织、肝硬化组织和肿瘤组织）的蛋白质组变化。使用二维聚丙烯酰胺凝胶电泳和基质辅助激光解吸/电离飞行时间质谱法观察蛋白质组变化。将这些组织类型相互比较，发现21种蛋白质的表达水平有显著变化。在这些蛋白质中，肌氨酸脱氢酶、肝脏羧酸酯酶、肽基脯氨酰异构酶A和核纤层蛋白B1被认为是新的HCC标志物候选物。特别是，核纤层蛋白B1可被视为肝硬化的标志物，因为与正常组织相比，其在肝硬化组织中的表达水平有相当大的变化。本实验中揭示的蛋白质未来可用于肝癌发生相关研究，或作为HCC的诊断标志物和治疗靶点。
