Ai Jianhua, Tan Yexiong, Ying Wantao, Hong Yi, Liu Shuqing, Wu Mengchao, Qian Xiaohong, Wang Hongyang
International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, the Second Military Medical University, Shanghai, PR China.
Proteomics. 2006 Jan;6(2):538-46. doi: 10.1002/pmic.200500257.
Hepatocellular carcinoma (HCC) is one of the most frequent visceral neoplasia worldwide and is a multifactorial and multistage pathogenesis that finally leads to the deregulation of cell homeostasis. Laser capture microdissection (LCM) may allow a more ready identification of differences in protein expression in selected cell types or areas of tissue, and microscopic regions as small as 3-5 microm in diameter can be sampled. Here we applied the LCM to the proteomic study of hepatitis B-related HCC and surrounding non-tumor tissues. Proteome alterations were observed using 2-DE and ESI-MS/MS, and alterations in the proteome were examined. Twenty protein spots were selected, of which 11 proteins were significantly altered in the HCC compared with the surrounding non-tumor tissues. Of the proteins that were selected, peroxiredoxin 2, apolipoprotein A-I precursor, 3-hydroxyacyl-CoA dehydrogenase type II, and 14.5-kDa translational inhibitor protein appear to be novel candidates as useful hepatitis B-related HCC markers. This study indicates that LCM is a useful technological method in the proteomic study of cancer tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC associated with hepatitis B virus infection.
肝细胞癌（HCC）是全球最常见的内脏肿瘤之一，其发病机制涉及多因素和多阶段，最终导致细胞内稳态失调。激光捕获显微切割（LCM）技术能够更便捷地识别特定细胞类型或组织区域中蛋白质表达的差异，甚至可以采集直径小至3 - 5微米的微观区域样本。在此，我们将LCM技术应用于乙肝相关HCC及周围非肿瘤组织的蛋白质组学研究。通过二维电泳（2-DE）和电喷雾串联质谱（ESI-MS/MS）观察蛋白质组的变化，并对蛋白质组的改变进行分析。我们选取了20个蛋白点，其中11种蛋白质在HCC组织与周围非肿瘤组织相比有显著变化。在所选择的蛋白质中，过氧化物还原酶2、载脂蛋白A-I前体、II型3-羟基酰基辅酶A脱氢酶以及14.5 kDa翻译抑制蛋白似乎是与乙肝相关的HCC有用标志物的新候选者。本研究表明，LCM是癌症组织蛋白质组学研究中一种有用的技术方法。本实验中揭示的蛋白质未来可用于肝癌发生机制的研究，或作为乙肝病毒感染相关HCC的诊断标志物和治疗靶点。
